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C3a Receptor antibody

KO/KD Orthogonal
Anti-C3a Receptor antibody used in Western Blot (WB). GTX114293
Anti-C3a Receptor antibody used in Western Blot (WB). GTX114293
Anti-C3a Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX114293
Anti-C3a Receptor antibody used in IHC (Frozen sections) (IHC-Fr). GTX114293
Anti-C3a Receptor antibody used in Western Blot (WB). GTX114293

Cat. No. GTX114293

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all C3a Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HL-60 nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C3a receptor 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169190

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

complement C3a receptor 1 , AZ3B , C3AR , HNFAG09

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-C3a Receptor antibody used in Western Blot (WB). GTX114293

GTX114293 WB Image

KO/KD Orthogonal

Non-transfected (–) and transfected (+) unboiled HL-60 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C3a Receptor antibody (GTX114293) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-C3a Receptor antibody used in Western Blot (WB). GTX114293

GTX114293 WB Image

Orthogonal

Boiled HL-60 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C3a Receptor antibody (GTX114293) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 12871936

Anti-C3a Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX114293

GTX114293 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using C3a receptor 1(GTX114293) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-C3a Receptor antibody used in IHC (Frozen sections) (IHC-Fr). GTX114293

GTX114293 IHC-Fr Image

The data was published in the journal PLoS One in 2014. PMID: 25422985

Anti-C3a Receptor antibody used in Western Blot (WB). GTX114293

GTX114293 WB Image

The data was published in the journal PLoS One in 2014. PMID: 25422985

REFERENCE

Application Reference

Li L et al. PLoS One 2014; 9 (11) : e113639 C3a Receptor Antagonist Ameliorates Inflammatory and Fibrotic Signals in Type 2 Diabetic Nephropathy by Suppressing the Activation of TGF-β/smad3 and IKBα Pathway.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169