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C4 binding protein B antibody

Anti-C4 binding protein B antibody used in Western Blot (WB). GTX104277
Anti-C4 binding protein B antibody used in IHC (Paraffin sections) (IHC-P). GTX104277
Anti-C4 binding protein B antibody used in Western Blot (WB). GTX104277

Cat No. GTX104277

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , BCL-1
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C4 binding protein B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

complement component 4 binding protein beta , C4BP

Cellular Localization

Secreted

Background

This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. A single, unique beta-chain encoded by this gene assembles with seven identical alpha-chains into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. C4b-binding protein has a regulatory role in the coagulation system also, mediated through the beta-chain binding of protein S, a vitamin K-dependent protein that serves as a cofactor of activated protein C. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Alternative splicing gives rise to multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-C4 binding protein B antibody used in Western Blot (WB). GTX104277

GTX104277 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: K562
12% SDS PAGE
GTX104277 diluted at 1:1000

Anti-C4 binding protein B antibody used in IHC (Paraffin sections) (IHC-P). GTX104277

GTX104277 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using C4 binding protein B(GTX104277) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-C4 binding protein B antibody used in Western Blot (WB). GTX104277

GTX104277 WB Image

Sample (30 ug of whole cell lysate)
A: BCL-1
12% SDS PAGE
GTX104277 diluted at 1:1000

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf