APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:100 |
| 1:50 - 1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1567-1676 of human C5 (NP_001726.2).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
complement C5 , C5D , C5a , C5b , CPAMD4 , ECLZB
Cellular Localization
Secreted
Background
This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the C5 alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mutations in this gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]
Database
Research Area
DATA IMAGES
|
GTX33052 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX33052 C5 / C5b antibody.
Blue : DAPI
Dilution : 1:100
|
|
|
GTX33052 WB Image
WB analysis of various sample lysates using GTX33052 C5 / C5b antibody.
Dilution : 1:1000
Loading : 25μg per lane
|
|
|
GTX33052 IHC-P Image
IHC-P analysis of human stomach tissue using GTX33052 C5 / C5b antibody.
Dilution : 1:100
|
|
|
GTX33052 WB Image
WB analysis of various sample lysates using GTX33052 C5 / C5b antibody.
Dilution : 1:1000
Loading : 25μg per lane
|
REFERENCE
There are currently no references for C5 / C5b antibody (GTX33052). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for C5 / C5b antibody (GTX33052). Be the first to share your experience with this product.
