C5L2 antibody [HL4135]

C5L2 antibody [HL4135] detects C5L2 protein by immunofluorescent analysis.
Sample: T-47D cells were fixed in ice-cold MeOH for 5 min.
Green: C5L2 stained by C5L2 antibody [HL4135] (GTX642940) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Boiled and unboiled T-47D whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C5L2 antibody [HL4135] (GTX642940) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C5L2 antibody [HL4135] (GTX642940) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.