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C5a R1 antibody [S5/1]

Anti-C5a R1 antibody [S5/1] used in IHC (Paraffin sections) (IHC-P). GTX74845
Anti-C5a R1 antibody [S5/1] used in Flow cytometry (FACS). GTX74845

Cat. No. GTX74845

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 S5/1

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS, Neutralizing/Inhibition

Reactivity

 Human, Rabbit, Bovine, Ferret, Mink
Package
100 μg ($399)
See all C5a R1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
FACS 1/50-1/100
Neutralizing/Inhibition Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 5 x 10 5 cells in 100μl.

Neutralizing/Inhibition
Clone S5/1 has been shown to inhibit the binding of C5a to its receptor.
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Product Note

This antibody recognizes the N-terminal extracellular domain of C5a R1 (met1-Asn31).

Predict Reactivity

Mustelid(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

C5aR - peptide: Met1 - Asn31.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_376501

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

complement C5a receptor 1 , C5A , C5AR , C5R1 , CD88

Cellular Localization

Cell membrane , Cytoplasmic vesicle

Database

Research Area

DATA IMAGES

Anti-C5a R1 antibody [S5/1] used in IHC (Paraffin sections) (IHC-P). GTX74845

GTX74845 IHC-P Image

IHC-P analysis of human lung tissue using GTX74845 C5a R1 antibody [S5/1].

Anti-C5a R1 antibody [S5/1] used in Flow cytometry (FACS). GTX74845

GTX74845 FACS Image

FACS analysis of human peripheral blood granulocytes using GTX74845 C5a R1 antibody [S5/1].

REFERENCE

Application Reference

Franke V et al. Life (Basel) 2022; 12 (10) : 1527 Complement Regulation in Immortalized Fibroblast-like Synoviocytes and Primary Human Endothelial Cells in Response to SARS-CoV-2 Nucleocapsid Protein and Pro-Inflammatory Cytokine TNFα
Application : ICC/IF
Reactivity : Human
Sandeep Silawal et al. Int J Mol Sci. 2021; 22 (6) : 3105 Complement Regulation in Human Tenocytes under the Influence of Anaphylatoxin C5a.
Application : ICC/IF
Reactivity : Human
Badendick J et al. Clin Med Insights Arthritis Musculoskelet Disord 2016; 9 : 65-74 Macroscopical, Histological, and In Vitro Characterization of Nonosteoarthritic Versus Osteoarthritic Hip Joint Cartilage.
Application : IHC-P
Reactivity : Human

Clone Reference

Cao Q et al. Am J Physiol Cell Physiol 2012; 302 (12):C1731-40 Human colonic epithelial cells detect and respond to C5a via apically expressed C5aR through the ERK pathway.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399