GeneTex
United States (US)

C5orf54 antibody

Anti-C5orf54 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123000
Anti-C5orf54 antibody used in IHC (Paraffin sections) (IHC-P). GTX123000
Anti-C5orf54 antibody used in Western Blot (WB). GTX123000

Cat No. GTX123000

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

H1299 , MCF-7

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human C5orf54. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zinc Finger Bed-Type Containing 8,Buster3,C5Orf54,Zbed8

Database

DATA IMAGES
Anti-C5orf54 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123000

GTX123000 ICC/IF Image

C5orf54 antibody detects C5orf54 protein at mitochondria by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C5orf54 protein stained by C5orf54 antibody (GTX123000) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-C5orf54 antibody used in IHC (Paraffin sections) (IHC-P). GTX123000

GTX123000 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using C5orf54(GTX123000) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-C5orf54 antibody used in Western Blot (WB). GTX123000

GTX123000 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: MCF-7
7.5% SDS PAGE
GTX123000 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169