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C6orf97 antibody

Anti-C6orf97 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107144
Anti-C6orf97 antibody used in IHC (Paraffin sections) (IHC-P). GTX107144
Anti-C6orf97 antibody used in Western Blot (WB). GTX107144
Anti-C6orf97 antibody used in IHC (Paraffin sections) (IHC-P). GTX107144

Cat No. GTX107144

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

A549

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human C6orf97. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036392

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

coiled-coil domain containing 170 , C6orf97 , bA282P11.1

Background

The function of this gene and its encoded protein is not known. Several genome-wide association studies have implicated the region around this gene to be involved in breast cancer and bone mineral density, but no link to this specific gene has been found. [provided by RefSeq]

Database

DATA IMAGES

Anti-C6orf97 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107144

GTX107144 ICC/IF Image

C6orf97 antibody detects C6orf97 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C6orf97 protein stained by C6orf97 antibody (GTX107144) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-C6orf97 antibody used in IHC (Paraffin sections) (IHC-P). GTX107144

GTX107144 IHC-P Image

C6orf97 antibody detects C6orf97 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
C6orf97 antibody (GTX107144) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-C6orf97 antibody used in Western Blot (WB). GTX107144

GTX107144 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX107144 diluted at 1:1000

Anti-C6orf97 antibody used in IHC (Paraffin sections) (IHC-P). GTX107144

GTX107144 IHC-P Image

C6orf97 antibody detects C6orf97 protein at cytoplasm in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
C6orf97 antibody (GTX107144) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

C6orf97 antibody Cat No. GTX107144

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2014. PMID: 25099679
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169