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C9 antibody [N2C2-2], Internal

Anti-C9 antibody [N2C2-2], Internal used in Western Blot (WB). GTX110161
Anti-C9 antibody [N2C2-2], Internal used in Western Blot (WB). GTX110161
Anti-C9 antibody [N2C2-2], Internal used in Western Blot (WB). GTX110161
Anti-C9 antibody [N2C2-2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110161
Anti-C9 antibody [N2C2-2], Internal used in Western Blot (WB). GTX110161

Cat. No. GTX110161

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all C9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

THP-1 , human plasma

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036638

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

complement C9 , ARMD15 , C9D

Cellular Localization

Membrane; Multi-pass membrane protein , Secreted , Membrane , Secreted

Background

This gene encodes the final component of the complement system. It participates in the formation of the Membrane Attack Complex (MAC). The MAC assembles on bacterial membranes to form a pore, permitting disruption of bacterial membrane organization. Mutations in this gene cause component C9 deficiency. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-C9 antibody [N2C2-2], Internal used in Western Blot (WB). GTX110161

GTX110161 WB Image

Sample (30 μg of whole cell lysate)
A: THP-1
7.5% SDS PAGE
GTX110161 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C9 antibody [N2C2-2], Internal used in Western Blot (WB). GTX110161

GTX110161 WB Image

Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C9 antibody [N2C2-2], Internal (GTX110161) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C9 antibody [N2C2-2], Internal used in Western Blot (WB). GTX110161

GTX110161 WB Image

Mouse plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C9 antibody [N2C2-2], Internal (GTX110161) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-C9 antibody [N2C2-2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110161

GTX110161 ICC/IF Image

C9 antibody [N2C2-2], Internal detects C9 protein at by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C9 stained by C9 antibody [N2C2-2], Internal (GTX110161) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-C9 antibody [N2C2-2], Internal used in Western Blot (WB). GTX110161

GTX110161 WB Image

Rat plasma (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with C9 antibody [N2C2-2], Internal (GTX110161) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169