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C9orf72 antibody [GT1553-RB]

Recombinant antibody KO/KD Overexpression
Anti-C9orf72 antibody [GT1553-RB] used in Western Blot (WB). GTX635398
Anti-C9orf72 antibody [GT1553-RB] used in IHC (Paraffin sections) (IHC-P). GTX635398
Anti-C9orf72 antibody [GT1553-RB] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635398
Anti-C9orf72 antibody [GT1553-RB] used in Western Blot (WB). GTX635398
Anti-C9orf72 antibody [GT1553-RB] used in Western Blot (WB). GTX635398
Anti-C9orf72 antibody [GT1553-RB] used in Western Blot (WB). GTX635398

Cat. No. GTX635398

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1553-RB

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all C9orf72 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human C9orf72. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888526

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C9orf72-SMCR8 complex subunit , ALSFTD , DENNL72 , FTDALS , FTDALS1

Cellular Localization

Nucleus,Cytoplasm,Endosome,Lysosome,Secreted,Cell projection, dendrite

Background

The protein encoded by this gene plays an important role in the regulation of endosomal trafficking, and has been shown to interact with Rab proteins that are involved in autophagy and endocytic transport. Expansion of a GGGGCC repeat from 2-22 copies to 700-1600 copies in the intronic sequence between alternate 5' exons in transcripts from this gene is associated with 9p-linked ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia) (PMID: 21944778, 21944779). Studies suggest that hexanucleotide expansions could result in the selective stabilization of repeat-containing pre-mRNA, and the accumulation of insoluble dipeptide repeat protein aggregates that could be pathogenic in FTD-ALS patients (PMID: 23393093). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-C9orf72 antibody [GT1553-RB] used in Western Blot (WB). GTX635398

GTX635398 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553-RB] (GTX635398) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C9orf72 antibody [GT1553-RB] used in IHC (Paraffin sections) (IHC-P). GTX635398

GTX635398 IHC-P Image

C9orf72 antibody [GT1553-RB] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
C9orf72 stained by C9orf72 antibody [GT1553-RB] (GTX635398) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C9orf72 antibody [GT1553-RB] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635398

GTX635398 ICC/IF Image

C9orf72 antibody [GT1553-RB] detects C9orf72 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C9orf72 stained by C9orf72 antibody [GT1553-RB] (GTX635398) diluted at 1:250.
Red: Tau, a cytoskeleton marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-C9orf72 antibody [GT1553-RB] used in Western Blot (WB). GTX635398

GTX635398 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553-RB] (GTX635398) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C9orf72 antibody [GT1553-RB] used in Western Blot (WB). GTX635398

GTX635398 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553-RB] (GTX635398) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C9orf72 antibody [GT1553-RB] used in Western Blot (WB). GTX635398

GTX635398 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553-RB] (GTX635398) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations