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C9orf72 antibody [GT1553]

Anti-C9orf72 antibody [GT1553] used in Western Blot (WB). GTX634482
Anti-C9orf72 antibody [GT1553] used in Western Blot (WB). GTX634482
Anti-C9orf72 antibody [GT1553] used in IHC (Paraffin sections) (IHC-P). GTX634482
Anti-C9orf72 antibody [GT1553] used in IHC (Paraffin sections) (IHC-P). GTX634482
Anti-C9orf72 antibody [GT1553] used in IHC (Paraffin sections) (IHC-P). GTX634482
Anti-C9orf72 antibody [GT1553] used in Western Blot (WB). GTX634482
Anti-C9orf72 antibody [GT1553] used in Western Blot (WB). GTX634482
Anti-C9orf72 antibody [GT1553] used in Western Blot (WB). GTX634482

Cat No. GTX634482

Host Mouse
Clonality Monoclonal
Clone Name GT1553
Isotype IgG2a
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , U87-MG , SK-N-SH , IMR32 , SK-N-AS , Neuro2A , NIH-3T3 , Raw264.7 , C2C12 , mouse brain , C9orf72-transfected 293T , shC9orf72-transfected 293T

Predict Reactivity

Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human C9orf72. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C9orf72-SMCR8 complex subunit,ALSFTD,DENNL72,FTDALS,FTDALS1

Cellular Localization

Nucleus,Cytoplasm,Endosome,Lysosome,Secreted,Cell projection, dendrite

Background

The protein encoded by this gene plays an important role in the regulation of endosomal trafficking, and has been shown to interact with Rab proteins that are involved in autophagy and endocytic transport. Expansion of a GGGGCC repeat from 2-22 copies to 700-1600 copies in the intronic sequence between alternate 5' exons in transcripts from this gene is associated with 9p-linked ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia) (PMID: 21944778, 21944779). Studies suggest that hexanucleotide expansions could result in the selective stabilization of repeat-containing pre-mRNA, and the accumulation of insoluble dipeptide repeat protein aggregates that could be pathogenic in FTD-ALS patients (PMID: 23393093). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES
Anti-C9orf72 antibody [GT1553] used in Western Blot (WB). GTX634482

GTX634482 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553] (GTX634482) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-C9orf72 antibody [GT1553] used in Western Blot (WB). GTX634482

GTX634482 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553] (GTX634482) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-C9orf72 antibody [GT1553] used in IHC (Paraffin sections) (IHC-P). GTX634482

GTX634482 IHC-P Image

C9orf72 antibody [GT1553] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
C9orf72 stained by C9orf72 antibody [GT1553] (GTX634482) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C9orf72 antibody [GT1553] used in IHC (Paraffin sections) (IHC-P). GTX634482

GTX634482 IHC-P Image

C9orf72 antibody [GT1553] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
C9orf72 stained by C9orf72 antibody [GT1553] (GTX634482) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C9orf72 antibody [GT1553] used in IHC (Paraffin sections) (IHC-P). GTX634482

GTX634482 IHC-P Image

C9orf72 antibody [GT1553] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
C9orf72 stained by C9orf72 antibody [GT1553] (GTX634482) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C9orf72 antibody [GT1553] used in Western Blot (WB). GTX634482

GTX634482 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553] (GTX634482) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-C9orf72 antibody [GT1553] used in Western Blot (WB). GTX634482

GTX634482 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553] (GTX634482) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-C9orf72 antibody [GT1553] used in Western Blot (WB). GTX634482

GTX634482 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553] (GTX634482) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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SDS
PBS.pdf