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C9orf72 antibody [GT779-RB]

Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397
Anti-C9orf72 antibody [GT779-RB] used in IHC (Paraffin sections) (IHC-P). GTX635397
Anti-C9orf72 antibody [GT779-RB] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635397
Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397
Anti-C9orf72 antibody [GT779-RB] used in IHC (Paraffin sections) (IHC-P). GTX635397
Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397

Cat No. GTX635397

Host

Rabbit

Clonality

Monoclonal

Clone Name

GT779-RB

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human C9orf72. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888525

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C9orf72-SMCR8 complex subunit , ALSFTD , DENNL72 , FTDALS , FTDALS1

Cellular Localization

Nucleus,Cytoplasm,Endosome,Lysosome,Secreted,Cell projection, dendrite

Background

The protein encoded by this gene plays an important role in the regulation of endosomal trafficking, and has been shown to interact with Rab proteins that are involved in autophagy and endocytic transport. Expansion of a GGGGCC repeat from 2-22 copies to 700-1600 copies in the intronic sequence between alternate 5' exons in transcripts from this gene is associated with 9p-linked ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia) (PMID: 21944778, 21944779). Studies suggest that hexanucleotide expansions could result in the selective stabilization of repeat-containing pre-mRNA, and the accumulation of insoluble dipeptide repeat protein aggregates that could be pathogenic in FTD-ALS patients (PMID: 23393093). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397

GTX635397 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779-RB] (GTX635397) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C9orf72 antibody [GT779-RB] used in IHC (Paraffin sections) (IHC-P). GTX635397

GTX635397 IHC-P Image

C9orf72 antibody [GT779-RB] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
C9orf72 stained by C9orf72 antibody [GT779-RB] (GTX635397) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C9orf72 antibody [GT779-RB] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635397

GTX635397 ICC/IF Image

C9orf72 antibody [GT779-RB] detects C9orf72 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C9orf72 stained by C9orf72 antibody [GT779-RB] (GTX635397) diluted at 1:250.
Red: Tau, a cytoskeleton marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397

GTX635397 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779-RB] (GTX635397) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C9orf72 antibody [GT779-RB] used in IHC (Paraffin sections) (IHC-P). GTX635397

GTX635397 IHC-P Image

C9orf72 antibody [GT779-RB] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
C9orf72 stained by C9orf72 antibody [GT779-RB] (GTX635397) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397

GTX635397 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779-RB] (GTX635397) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for C9orf72 antibody [GT779-RB] (GTX635397). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 379
$ 169
Custom Conjugations