C9orf72 antibody [GT779]

Name: C9orf72 antibody [GT779]
Brand: GeneTex
SKU: GTX632041
Price: 329.00 USD
Rating: 5 (1 reviews)

Anti-C9orf72 antibody [GT779] used in Western Blot (WB). GTX632041

Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041

Anti-C9orf72 antibody [GT779] used in Immunoprecipitation (IP). GTX632041

Anti-C9orf72 antibody [GT779] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632041

Cat No. GTX632041

Reference 2

Host	Mouse
Clonality	Monoclonal
Clone Name	GT779
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($329), 25 μl ($169)

See all C9orf72 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain

Product Note

Knockdown/Knockout validation was supported by customer review data

Predict Reactivity

Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human C9orf72.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2784546

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C9orf72-SMCR8 complex subunit , ALSFTD , DENNL72 , FTDALS , FTDALS1

Database

Gene ID: 203228 C9orf72
UniProt: Q96LT7 C9orf72

Research Area

DATA IMAGES

GTX632041 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (GTX632041) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX632041 IHC-P Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
C9orf72 stained by C9orf72 antibody [GT779] (GTX632041) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632041 IHC-P Image

GTX632041 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (GTX632041) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX632041 IP Image

Immunoprecipitation of C9orf72 protein from SK-N-SH whole cell extracts using 5 μg of C9orf72 antibody [GT779] (GTX632041).
Western blot analysis was performed using C9orf72 antibody [GT779] (GTX632041).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX632041 IHC-P Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
C9orf72 antibody [GT779] (GTX632041) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632041 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody (GTX632041) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX632041 IHC-P Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
C9orf72 stained by C9orf72 antibody [GT779] (GTX632041) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632041 IHC-P Image

GTX632041 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (GTX632041) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX632041 ICC/IF Image

C9orf72 antibody detects C9orf72 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C9orf72 stained by C9orf72 antibody (GTX632041) diluted at 1:500.
Red: NeuN , a marker, stained by NeuN antibody (GTX132974) diluted at 1:500.

REFERENCE

Application Reference

Laflamme C et al. Elife 2019; 8 Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72.

Application : WB, ICC/IF, IP

Reactivity : Human

Carl Laflamme et al. bioRxiv 2018; (Epub) Implementation of an antibody characterization process: Application to the major ALS/FTD disease gene C9ORF72.

Application : WB, ICC/IF, IP

Reactivity : Human, Mouse

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