C9orf72 antibody [GT779]

Name: C9orf72 antibody [GT779]
Brand: GeneTex
SKU: GTX632041
Price: 379.00 USD
Rating: 5 (1 reviews)

Anti-C9orf72 antibody [GT779] used in Western Blot (WB). GTX632041

Anti-C9orf72 antibody [GT779] used in Immunoprecipitation (IP). GTX632041

Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041

Anti-C9orf72 antibody [GT779] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632041

Cat No. GTX632041

Reference 3

Host	Mouse
Clonality	Monoclonal
Clone Name	GT779
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($379), 25 μl ($169)

See all C9orf72 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain

Product Note

Knockdown/Knockout validation was supported by customer review data

Predict Reactivity

Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human C9orf72. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2784546

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C9orf72-SMCR8 complex subunit , ALSFTD , DENNL72 , FTDALS , FTDALS1

Database

Gene ID: 203228 C9orf72
UniProt: Q96LT7 C9orf72

Research Area

DATA IMAGES

GTX632041 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (GTX632041) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX632041 IP Image

Immunoprecipitation of C9orf72 protein from SK-N-SH whole cell extracts using 5 μg of C9orf72 antibody [GT779] (GTX632041).
Western blot analysis was performed using C9orf72 antibody [GT779] (GTX632041).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX632041 IHC-P Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
C9orf72 antibody [GT779] (GTX632041) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632041 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody (GTX632041) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX632041 IHC-P Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
C9orf72 stained by C9orf72 antibody [GT779] (GTX632041) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632041 IHC-P Image

GTX632041 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (GTX632041) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX632041 IHC-P Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
C9orf72 stained by C9orf72 antibody [GT779] (GTX632041) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632041 IHC-P Image

GTX632041 ICC/IF Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C9orf72 stained by C9orf72 antibody [GT779] (GTX632041) diluted at 1:500.
Red: Tau, a Tau marker, stained by Tau antibody (GTX130462) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX632041 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (GTX632041) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Liu Y et al. Nat Commun. 2021; 12 (1) : 847 Variant-selective stereopure oligonucleotides protect against pathologies associated with C9orf72-repeat expansion in preclinical models.

Application : WB

Reactivity : Human

Laflamme C et al. Elife 2019; 8 Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72.

Application : WB, ICC/IF, IP

Reactivity : Human

Carl Laflamme et al. bioRxiv 2018; (Epub) Implementation of an antibody characterization process: Application to the major ALS/FTD disease gene C9ORF72.

Application : WB, ICC/IF, IP

Reactivity : Human, Mouse

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