GeneTex
United States (US)

C9orf72 antibody

GTX119776_40884_20150512_IHC_18121410_296.jpg
GTX119776_40352_IHC_M_18121410_326.jpg
GTX119776_40352_WB_M_18121410_575.jpg
GTX119776_40884_WB_B_18121410_633.jpg

Cat No. GTX119776

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Neuro2A , C9orf72 transfected 293T lysate

Predict Reactivity

Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C9orf72. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Chromosome 9 Open Reading Frame 72,Alsftd,Dennl72,Ftdals,Ftdals1,C9Orf72

Database

Research Area

DATA IMAGES
GTX119776_40884_20150512_IHC_18121410_296.jpg

GTX119776 IHC-P Image

C9orf72 antibody detects C9orf72 protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
C9orf72 antibody (GTX119776) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX119776_40352_IHC_M_18121410_326.jpg

GTX119776 IHC-P Image

C9orf72 antibody detects C9orf72 protein at on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
C9orf72 antibody (GTX119776) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX119776_40352_WB_M_18121410_575.jpg

GTX119776 WB Image

Sample (30 µg of whole cell lysate)
A: Neuro2A
10% SDS PAGE
GTX119776 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX119776_40884_WB_B_18121410_633.jpg

GTX119776 WB Image

Sample (30 µg of whole cell lysate)
A: Non-transfected 293T lysates
B: C9orf72 transfected 293T lysate
10% SDS PAGE
GTX119776 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169
Current in productions