GeneTex
United States (US)

C9orf72 antibody

GTX119776_40884_WB_B_18121410_633.jpg
GTX119776_40884_20150512_IHC_18121410_296.jpg
GTX119776_40352_IHC_M_18121410_326.jpg
GTX119776_40352_WB_M_18121410_575.jpg

Cat No. GTX119776

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Neuro2A , C9orf72 transfected 293T lysate

Predict Reactivity

Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C9orf72. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC23980 antibody, RP11-27J8.2 antibody, C9orf72 antibody, uncharacterized protein C9orf72 antibody, chromosome 9 open reading frame 72 antibody

Database

Research Area

DATA IMAGES
GTX119776_40884_WB_B_18121410_633.jpg

GTX119776 WB Image

Sample (30 µg of whole cell lysate)
A: Non-transfected 293T lysates
B: C9orf72 transfected 293T lysate
10% SDS PAGE
GTX119776 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX119776_40884_20150512_IHC_18121410_296.jpg

GTX119776 IHC-P Image

C9orf72 antibody detects C9orf72 protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
C9orf72 antibody (GTX119776) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX119776_40352_IHC_M_18121410_326.jpg

GTX119776 IHC-P Image

C9orf72 antibody detects C9orf72 protein at on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
C9orf72 antibody (GTX119776) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX119776_40352_WB_M_18121410_575.jpg

GTX119776 WB Image

Sample (30 µg of whole cell lysate)
A: Neuro2A
10% SDS PAGE
GTX119776 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf