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United States (US)

C9orf78 antibody [N1C2]

Anti-C9orf78 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106966
Anti-C9orf78 antibody [N1C2] used in Western Blot (WB). GTX106966
Anti-C9orf78 antibody [N1C2] used in Western Blot (WB). GTX106966
Anti-C9orf78 antibody [N1C2] used in Western Blot (WB). GTX106966
Anti-C9orf78 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX106966

Cat No. GTX106966

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C9orf78. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Chromosome 9 Open Reading Frame 78,Hca59,Hspc220,Ba409K20.3,C9Orf78

Database

DATA IMAGES
Anti-C9orf78 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106966

GTX106966 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using C9orf78(GTX106966) antibody at 1:200 dilution.

Anti-C9orf78 antibody [N1C2] used in Western Blot (WB). GTX106966

GTX106966 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with C9orf78 antibody [N1C2] (GTX106966) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C9orf78 antibody [N1C2] used in Western Blot (WB). GTX106966

GTX106966 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with C9orf78 antibody [N1C2] (GTX106966) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C9orf78 antibody [N1C2] used in Western Blot (WB). GTX106966

GTX106966 WB Image

Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3(GTX14654)
C:Hep G2 (GTX27900)
12% SDS PAGE
GTX106966 diluted at 1:3000

Anti-C9orf78 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX106966

GTX106966 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using C9orf78(GTX106966) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169