Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3(GTX14654)
C:Hep G2 (GTX27900)
12% SDS PAGE
GTX106966 diluted at 1:3000

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with C9orf78 antibody [N1C2] (GTX106966) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using C9orf78(GTX106966) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with C9orf78 antibody [N1C2] (GTX106966) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using C9orf78(GTX106966) antibody at 1:200 dilution.