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CA125 antibody [OV185:1]

Anti-CA125 antibody [OV185:1] used in IHC (Paraffin sections) (IHC-P). GTX20697

Cat. No. GTX20697

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 OV185:1

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
500 μl ($399)
See all CA125 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:25-1:50

Note :

IHC-P
Recommendations for antigen retrieval : Citrate Buffer pH 6.0 at room temperature for 30 mins.
Not tested in other applications.

Calculated MW

1519 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Partially purified human mucin fraction from a pool of cancer tissues of patients with epithelial ovarian cancer.

Purification

Purified IgG

Conjugation

Unconjugated

RRID

AB_384363

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mucin 16, cell surface associated , CA125

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a protein that is a member of the mucin family. Mucins are high molecular weight, O-glycosylated proteins that play an important role in forming a protective mucous barrier, and are found on the apical surfaces of the epithelia. The encoded protein is a membrane-tethered mucin that contains an extracellular domain at its amino terminus, a large tandem repeat domain, and a transmembrane domain with a short cytoplasmic domain. The amino terminus is highly glycosylated, while the repeat region contains 156 amino acid repeats unit that are rich in serines, threonines, and prolines. Interspersed within the repeats are Sea urchin sperm protein Enterokinase and Agrin (SEA) modules, leucine-rich repeats and ankyrin (ANK) repeats. These regions together form the ectodomain, and there is a potential cleavage site found near an SEA module close to the transmembrane domain. This protein is thought to play a role in forming a barrier, protecting epithelial cells from pathogens. Products of this gene have been used as a marker for different cancers, with higher expression levels associated with poorer outcomes. [provided by RefSeq, May 2017]

Database

Research Area

DATA IMAGES

Anti-CA125 antibody [OV185:1] used in IHC (Paraffin sections) (IHC-P). GTX20697

GTX20697 IHC-P Image

IHC-P analysis of human ovarian carcinoma tissue using GTX20697 CA125 antibody [OV185:1].

REFERENCE

Application Reference

Tchedre K et al. Eye Contact Lens 2013; 39 (6) : 394-9 Contact lens care solutions downregulate membrane-associated mucins 1 and 16 in cultured human corneal epithelial cells and at the rat corneal surface in vivo.
Application : WB, IHC-P
Reactivity : Rat
Tchedre KT et al. Eye Contact Lens 2011; 37 (5) : 328-30 Assessment of effects of multipurpose contact lens care solutions on human corneal epithelial cells.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399