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CA125 antibody

Anti-CA125 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03800
Anti-CA125 antibody used in IHC (Paraffin sections) (IHC-P). GTX03800
Anti-CA125 antibody used in Western Blot (WB). GTX03800

Cat. No. GTX03800

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($419)
See all CA125 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:3000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

1519 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human MUC16(Accession Q8WXI7), corresponding to amino acid residues T14477-Q14498.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mucin 16, cell surface associated , CA125

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a protein that is a member of the mucin family. Mucins are high molecular weight, O-glycosylated proteins that play an important role in forming a protective mucous barrier, and are found on the apical surfaces of the epithelia. The encoded protein is a membrane-tethered mucin that contains an extracellular domain at its amino terminus, a large tandem repeat domain, and a transmembrane domain with a short cytoplasmic domain. The amino terminus is highly glycosylated, while the repeat region contains 156 amino acid repeats unit that are rich in serines, threonines, and prolines. Interspersed within the repeats are Sea urchin sperm protein Enterokinase and Agrin (SEA) modules, leucine-rich repeats and ankyrin (ANK) repeats. These regions together form the ectodomain, and there is a potential cleavage site found near an SEA module close to the transmembrane domain. This protein is thought to play a role in forming a barrier, protecting epithelial cells from pathogens. Products of this gene have been used as a marker for different cancers, with higher expression levels associated with poorer outcomes. [provided by RefSeq, May 2017]

Database

Research Area

DATA IMAGES

Anti-CA125 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03800

GTX03800 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX03800 CA125 antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : Triton X-100 (0.1%, 45 min)
Dilution : 1:200

Anti-CA125 antibody used in IHC (Paraffin sections) (IHC-P). GTX03800

GTX03800 IHC-P Image

IHC-P analysis of mouse colon tissue using GTX03800 CA125 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-CA125 antibody used in Western Blot (WB). GTX03800

GTX03800 WB Image

WB analysis of NIH-3T3 cell lysates using GTX03800 CA125 antibody.

REFERENCE

Application Reference

Liu Y et al. Am J Physiol Cell Physiol 2024; 327 (4) : C1125-C1142 Hypomethylation-associated ELF3 helps nasopharyngeal carcinoma to escape immune surveillance via MUC16-mediated glycolytic metabolic reprogramming.
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REVIEW

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Package List Price ($)
$ 419