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CA150 antibody

Orthogonal
Anti-CA150 antibody used in IHC (Paraffin sections) (IHC-P). GTX112099
Anti-CA150 antibody used in Western Blot (WB). GTX112099
Anti-CA150 antibody used in Western Blot (WB). GTX112099
Anti-CA150 antibody used in IHC (Paraffin sections) (IHC-P). GTX112099
Anti-CA150 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112099
Anti-CA150 antibody used in IHC (Paraffin sections) (IHC-P). GTX112099

Cat. No. GTX112099

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CA150 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

U87-MG nuclear extract , HeLa nuclear extract

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CA150. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11178927

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

transcription elongation regulator 1 , CA150 , TAF2S , Urn1

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Alternative splicing results in multiple transcripts variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CA150 antibody used in IHC (Paraffin sections) (IHC-P). GTX112099

GTX112099 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon, using CA150(GTX112099) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CA150 antibody used in Western Blot (WB). GTX112099

GTX112099 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CA150 antibody (GTX112099) diluted at 1:500.

Anti-CA150 antibody used in Western Blot (WB). GTX112099

GTX112099 WB Image

Orthogonal

U87-MG whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CA150 antibody (GTX112099) diluted at 1:500.

Anti-CA150 antibody used in IHC (Paraffin sections) (IHC-P). GTX112099

GTX112099 IHC-P Image

CA150 antibody detects CA150 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CA150 antibody (GTX112099) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CA150 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112099

GTX112099 ICC/IF Image

CA150 antibody detects CA150 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CA150 protein stained by CA150 antibody (GTX112099) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Anti-CA150 antibody used in IHC (Paraffin sections) (IHC-P). GTX112099

GTX112099 IHC-P Image

CA150 antibody detects CA150 protein at nucleus in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
CA150 antibody (GTX112099) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169