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CA150 antibody

Orthogonal
Anti-CA150 antibody used in IHC (Paraffin sections) (IHC-P). GTX112099
Anti-CA150 antibody used in Western Blot (WB). GTX112099
Anti-CA150 antibody used in Western Blot (WB). GTX112099
Anti-CA150 antibody used in IHC (Paraffin sections) (IHC-P). GTX112099
Anti-CA150 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112099
Anti-CA150 antibody used in IHC (Paraffin sections) (IHC-P). GTX112099

Cat. No. GTX112099

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CA150 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

U87-MG nuclear extract , HeLa nuclear extract

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CA150. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11178927

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transcription elongation regulator 1 , CA150 , TAF2S , Urn1

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Alternative splicing results in multiple transcripts variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CA150 antibody used in IHC (Paraffin sections) (IHC-P). GTX112099

GTX112099 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon, using CA150(GTX112099) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CA150 antibody used in Western Blot (WB). GTX112099

GTX112099 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CA150 antibody (GTX112099) diluted at 1:500.

Anti-CA150 antibody used in Western Blot (WB). GTX112099

GTX112099 WB Image

Orthogonal

U87-MG whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CA150 antibody (GTX112099) diluted at 1:500.

Anti-CA150 antibody used in IHC (Paraffin sections) (IHC-P). GTX112099

GTX112099 IHC-P Image

CA150 antibody detects CA150 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CA150 antibody (GTX112099) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CA150 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112099

GTX112099 ICC/IF Image

CA150 antibody detects CA150 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CA150 protein stained by CA150 antibody (GTX112099) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Anti-CA150 antibody used in IHC (Paraffin sections) (IHC-P). GTX112099

GTX112099 IHC-P Image

CA150 antibody detects CA150 protein at nucleus in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
CA150 antibody (GTX112099) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169