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CA19-9 antibody [GT933]

Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389
Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389
Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389
Anti-CA19-9 antibody [GT933] used in Glycan Array (Glycan Array). GTX635389
Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389
Anti-CA19-9 antibody [GT933] used in Western Blot (WB). GTX635389
Anti-CA19-9 antibody [GT933] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635389
Anti-CA19-9 antibody [GT933] used in Flow cytometry (FACS). GTX635389
Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389
Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389

Cat No. GTX635389

Host

Mouse

Clonality

Monoclonal

Clone Name

GT933

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, ELISA, Sandwich ELISA, Glycan Array

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)
PRODUCT

Summary

Carbohydrate antigen 19-9 (CA 19-9), also known as sialyl-LewisA, is a tetrasaccharide with the sequence Neu5Acα2-3Galβ1-3[Fucα1-4]GlcNAcβ. It is used clinically to assist in the diagnosis of pancreatic cancer, but is more widely utilized to monitor therapy and detect recurrences in diagnosed cases. CA 19-9 is also elevated in many other diseases, including cholangiocarcinoma, colorectal cancer, hepatocellular carcinoma, and cirrhosis. GeneTex’s CA 19-9 mouse monoclonal antibody [GT933] demonstrates superior sensitivity for western blot, immunohistochemistry, and immunocytochemistry in comparative testing against other well-known CA 19-9 antibodies. Most significantly, glycan array interrogation using this antibody establishes that it is specific for sialyl-LewisA.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
Glycan Array Assay dependent

Note :

Sandwich ELISA
Capture: GTX635389, Detection: GTX635391 or Capture: GTX635391, Detection: GTX635389 Please notice that GTX635389 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635391. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Positive Control

SW1116 , HT29

Specificity/Sensitivity

This antibody specifically detects sialyl-Lewis A (CA 19-9), but not sialyl-Lewis X.

PROPERTIES

Form

Liquid

Buffer

20% Glycerol in PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to CA19-9.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389

GTX635389 IHC-P Image

CA19-9 antibody [GT933] detects CA19-9 by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic ductal adenocarcinoma.
CA19-9 stained by CA19-9 antibody [GT933] (GTX635389) diluted at 1:500 and competitor's antibody (Company A) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389

GTX635389 IHC-P Image

CA19-9 antibody [GT933] detects CA19-9 by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic ductal adenocarcinoma.
CA19-9 stained by CA19-9 antibody [GT933] (GTX635389) diluted at 1:500 and competitor's antibody diluted at 1:250 (Company A) and 1:500 (Company B).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389

GTX635389 IHC-P Image

CA19-9 antibody [GT933] detects CA19-9 by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic ductal adenocarcinoma.
CA19-9 stained by CA19-9 antibody [GT933] (GTX635389) diluted at 1:500 and competitor's antibody diluted at 1:250 (Company A) and 1:500 (Company B).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Anti-CA19-9 antibody [GT933] used in Glycan Array (Glycan Array). GTX635389

GTX635389 Glycan Array Image

CA19-9 antibody [GT933] specifically detects sialyl-LewisA (Neu5Ac-; CA19-9) on a glycan array.
Green: sialyl-LewisA (Neu5Ac-; CA19-9).
Red: Modified sialyl-LewisA (Neu5Gc-).
Yellow: Positive control.
Blue: Negative control.

Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389

GTX635389 ELISA Image

Competition analysis of CA 19-9 antibody [GT933] (GTX635389) (330 ng/mL) binding to immobilized recombinant HSA-CA 19-9 (coated at 3 μg/mL) in the presence of soluble LewisA or LewisX tetrasaccharides (1.0-0.01 mM). Bound antibody was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
Competition analysis of CA 19-9 antibody [GT933] (GTX635389) (330 ng/mL) binding to immobilized recombinant Isospec HAS-CA 19-9 (coated at 3 μg/mL) in the presence of soluble LewisA or LewisX tetrasaccharides (1.0-0.01 mM). Bound antibody was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Anti-CA19-9 antibody [GT933] used in Western Blot (WB). GTX635389

GTX635389 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CA19-9 antibody [GTX933] (GTX635389) diluted at 1:1000 and competitor's antibody (MA5-12421) diluted at 1:250. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-CA19-9 antibody [GT933] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635389

GTX635389 ICC/IF Image

CA19-9 antibody [GT933] detects CA19-9 protein at cell membrane by immunofluorescent analysis.
Sample: SW1116 (high expression) and HT29 (low expression) cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CA19-9 stained by CA19-9 antibody [GT933] (GTX635389) diluted at 1:1000 and competitor's antibody (MA5-12421 and M66107M) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
The observed expression level is based on the publication: PMID: 10212226

Anti-CA19-9 antibody [GT933] used in Flow cytometry (FACS). GTX635389

GTX635389 FACS Image

CA19-9 antibody [GT933] (GTX635389) detects CA19-9 by flow cytometry analysis.
Sample: COLO 205 cell.
Black: Unlabelled sample was used as a control.
Red: CA19-9 antibody [GT933] (GTX635389) dilution: 1:50.
Blue: Highly cited antibody [116-NS-19-9] dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389

GTX635389 ELISA Image

Sandwich ELISA detection of Colo 205 and Jurkat whole cell extracts using CA19-9 antibody [GT8510] (GTX635391) as capture antibody at concentration of 5 μg/mL and HRP-conjugated CA19-9 antibody [GT933] (GTX635389) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635389 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635391. Please contact us for custom HRP-conjugated antibody.

Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389

GTX635389 ELISA Image

Sandwich ELISA detection of Colo 205 and Jurkat whole cell extracts using CA19-9 antibody [GTX933] (GTX635389) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Ca19-9 antibody [GT8510] (GTX635391) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635391 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635389. Please contact us for custom HRP-conjugated antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 329
$ 169