PRODUCT
Summary
Carbohydrate antigen 19-9 (CA 19-9), also known as sialyl-LewisA, is a tetrasaccharide with the sequence Neu5Acα2-3Galβ1-3[Fucα1-4]GlcNAcβ. It is used clinically to assist in the diagnosis of pancreatic cancer, but is more widely utilized to monitor therapy and detect recurrences in diagnosed cases. CA 19-9 is also elevated in many other diseases, including cholangiocarcinoma, colorectal cancer, hepatocellular carcinoma, and cirrhosis. GeneTex’s CA 19-9 mouse monoclonal antibody [GT933] demonstrates superior sensitivity for western blot, immunohistochemistry, and immunocytochemistry in comparative testing against other well-known CA 19-9 antibodies. Most significantly, glycan array interrogation using this antibody establishes that it is specific for sialyl-LewisA.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1000 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
|
Note :
Sandwich ELISA
Capture: GTX635389, Detection: GTX635391 or Capture: GTX635391, Detection: GTX635389
Please notice that GTX635389 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635391. Please contact us for custom HRP-conjugated antibody.
|
Not tested in other applications.
Positive Control
SW1116 , HT29
Product Note
This antibody specifically detects sialyl-Lewis A (CA 19-9), but not sialyl-Lewis X.
PROPERTIES
Form
Liquid
Buffer
20% Glycerol in PBS
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
The immunogen used to generate this antibody corresponds to CA19-9.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888518
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
![Anti-CA19-9 antibody [GT933] used in Glycan Array (Glycan Array). GTX635389](/upload/website/prouct_img/middle/GTX635389/GTX635389_43899_20200820_Glycanarray_competitor_watermark_20081920_354.jpg "Anti-CA19-9 antibody [GT933] used in Glycan Array (Glycan Array). GTX635389")
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GTX635389 Glycan Array Image
CA19-9 antibody [GT933] specifically detects sialyl-LewisA (Neu5Ac-; CA19-9) on a glycan array. Green: sialyl-LewisA (Neu5Ac-; CA19-9). Red: Modified sialyl-LewisA (Neu5Gc-). Yellow: Positive control. Blue: Negative control.
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![Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389](/upload/website/prouct_img/middle/GTX635389/GTX635389_43899_20200821_Neutralizing_Inhibition_20083022_480.jpg "Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389")
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GTX635389 ELISA Image
Competition analysis of CA 19-9 antibody [GT933] (GTX635389) (330 ng/mL) binding to immobilized recombinant HSA-CA 19-9 (coated at 3 μg/mL) in the presence of soluble LewisA or LewisX tetrasaccharides (1.0-0.01 mM). Bound antibody was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
Competition analysis of CA 19-9 antibody [GT933] (GTX635389) (330 ng/mL) binding to immobilized recombinant Isospec HAS-CA 19-9 (coated at 3 μg/mL) in the presence of soluble LewisA or LewisX tetrasaccharides (1.0-0.01 mM). Bound antibody was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
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![Anti-CA19-9 antibody [GT933] used in Western Blot (WB). GTX635389](/upload/website/prouct_img/middle/GTX635389/GTX635389_43899_20200917_WB_competitor_watermark_20092220_853.jpg "Anti-CA19-9 antibody [GT933] used in Western Blot (WB). GTX635389")
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GTX635389 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CA19-9 antibody [GTX933] (GTX635389) diluted at 1:1000 and competitor's antibody (MA5-12421) diluted at 1:250. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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![Anti-CA19-9 antibody [GT933] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635389](/upload/website/prouct_img/middle/GTX635389/GTX635389_43899_20200519_ICC_IF_competitor_20061020_424.jpg "Anti-CA19-9 antibody [GT933] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635389")
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GTX635389 ICC/IF Image
CA19-9 antibody [GT933] detects CA19-9 protein at cell membrane by immunofluorescent analysis.Sample: SW1116 (high expression) and HT29 (low expression) cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CA19-9 stained by CA19-9 antibody [GT933] (GTX635389) diluted at 1:1000 and competitor's antibody (MA5-12421 and M66107M) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920). *Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
The observed expression level is based on the publication: PMID: 10212226
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![Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389](/upload/website/prouct_img/middle/GTX635389/GTX635389_43899_20201023_ELISA_PAIR_20110422_360.jpg "Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389")
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GTX635389 ELISA Image
Sandwich ELISA detection of Colo 205 and Jurkat whole cell extracts using CA19-9 antibody [GT8510] (GTX635391) as capture antibody at concentration of 5 μg/mL and HRP-conjugated CA19-9 antibody [GT933] (GTX635389) as detection antibody at concentration of 1 μg/mL.Please notice that GTX635389 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635391. Please contact us for custom HRP-conjugated antibody.
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![Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389](/upload/website/prouct_img/middle/GTX635389/GTX635389_43899_20201022_ELISA_PAIR_20110422_905.jpg "Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389")
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GTX635389 ELISA Image
Sandwich ELISA detection of Colo 205 and Jurkat whole cell extracts using CA19-9 antibody [GTX933] (GTX635389) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Ca19-9 antibody [GT8510] (GTX635391) as detection antibody at concentration of 1 μg/mL.Please notice that GTX635391 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635389. Please contact us for custom HRP-conjugated antibody.
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![Anti-CA19-9 antibody [GT933] used in Flow cytometry (FACS). GTX635389](/upload/website/prouct_img/middle/GTX635389/GTX635389_43899_20200617_FACS_20062300_389.jpg "Anti-CA19-9 antibody [GT933] used in Flow cytometry (FACS). GTX635389")
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GTX635389 FACS Image
CA19-9 antibody [GT933] (GTX635389) detects CA19-9 by flow cytometry analysis.
Sample: COLO 205 cell.
Black: Unlabelled sample was used as a control.
Red: CA19-9 antibody [GT933] (GTX635389) dilution: 1:50.
Blue: Highly cited antibody [116-NS-19-9] dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.
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![Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389](/upload/website/prouct_img/middle/GTX635389/GTX635389_43899_20210428_IHC-P_competitor_2_21050419_189.jpg "Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389")
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GTX635389 IHC-P Image
CA19-9 antibody [GTX933] detects CA19-9 by immunohistochemical analysis.Sample: Paraffin-embedded human pancreatic ductal adenocarcinoma.CA19-9 stained by CA19-9 antibody [GTX933] (GTX635389) diluted at 1:500 and competitor's antibody diluted at 1:250 (Company A) and 1:500 (Company B).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min *Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
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![Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389](/upload/website/prouct_img/middle/GTX635389/GTX635389_43899_20210428_IHC-P_competitor_3_21050419_192.jpg "Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389")
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GTX635389 IHC-P Image
CA19-9 antibody [GTX933] detects CA19-9 by immunohistochemical analysis.Sample: Paraffin-embedded human pancreatic ductal adenocarcinoma.CA19-9 stained by CA19-9 antibody [GTX933] (GTX635389) diluted at 1:500 and competitor's antibody (Company A) diluted at 1:250.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min *Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
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![Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389](/upload/website/prouct_img/middle/GTX635389/GTX635389_43899_20210428_IHC-P_competitor_1_21050419_587.jpg "Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389")
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GTX635389 IHC-P Image
CA19-9 antibody [GTX933] detects CA19-9 by immunohistochemical analysis.Sample: Paraffin-embedded human pancreatic ductal adenocarcinoma.CA19-9 stained by CA19-9 antibody [GTX933] (GTX635389) diluted at 1:500 and competitor's antibody diluted at 1:250 (Company A) and 1:500 (Company B).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min *Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
|
REFERENCE
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REVIEW
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![Anti-CA19-9 antibody [GT933] used in Glycan Array (Glycan Array). GTX635389](/upload/website/prouct_img/small/GTX635389/GTX635389_43899_20200820_Glycanarray_competitor_watermark_20081920_354.jpg "Anti-CA19-9 antibody [GT933] used in Glycan Array (Glycan Array). GTX635389")
![Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389](/upload/website/prouct_img/small/GTX635389/GTX635389_43899_20200821_Neutralizing_Inhibition_20083022_480.jpg "Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389")
![Anti-CA19-9 antibody [GT933] used in Western Blot (WB). GTX635389](/upload/website/prouct_img/small/GTX635389/GTX635389_43899_20200917_WB_competitor_watermark_20092220_853.jpg "Anti-CA19-9 antibody [GT933] used in Western Blot (WB). GTX635389")
![Anti-CA19-9 antibody [GT933] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635389](/upload/website/prouct_img/small/GTX635389/GTX635389_43899_20200519_ICC_IF_competitor_20061020_424.jpg "Anti-CA19-9 antibody [GT933] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635389")
![Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389](/upload/website/prouct_img/small/GTX635389/GTX635389_43899_20201023_ELISA_PAIR_20110422_360.jpg "Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389")
![Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389](/upload/website/prouct_img/small/GTX635389/GTX635389_43899_20201022_ELISA_PAIR_20110422_905.jpg "Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389")
![Anti-CA19-9 antibody [GT933] used in Flow cytometry (FACS). GTX635389](/upload/website/prouct_img/small/GTX635389/GTX635389_43899_20200617_FACS_20062300_389.jpg "Anti-CA19-9 antibody [GT933] used in Flow cytometry (FACS). GTX635389")
![Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389](/upload/website/prouct_img/small/GTX635389/GTX635389_43899_20210428_IHC-P_competitor_2_21050419_189.jpg "Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389")
![Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389](/upload/website/prouct_img/small/GTX635389/GTX635389_43899_20210428_IHC-P_competitor_3_21050419_192.jpg "Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389")
![Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389](/upload/website/prouct_img/small/GTX635389/GTX635389_43899_20210428_IHC-P_competitor_1_21050419_587.jpg "Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389")
![Anti-CA19-9 antibody [GT933] used in Glycan Array (Glycan Array). GTX635389](/upload/website/prouct_img/normal/GTX635389/GTX635389_43899_20200820_Glycanarray_competitor_watermark_20081920_354.jpg "Anti-CA19-9 antibody [GT933] used in Glycan Array (Glycan Array). GTX635389")
![Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389](/upload/website/prouct_img/normal/GTX635389/GTX635389_43899_20200821_Neutralizing_Inhibition_20083022_480.jpg "Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389")
![Anti-CA19-9 antibody [GT933] used in Western Blot (WB). GTX635389](/upload/website/prouct_img/normal/GTX635389/GTX635389_43899_20200917_WB_competitor_watermark_20092220_853.jpg "Anti-CA19-9 antibody [GT933] used in Western Blot (WB). GTX635389")
![Anti-CA19-9 antibody [GT933] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635389](/upload/website/prouct_img/normal/GTX635389/GTX635389_43899_20200519_ICC_IF_competitor_20061020_424.jpg "Anti-CA19-9 antibody [GT933] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635389")
![Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389](/upload/website/prouct_img/normal/GTX635389/GTX635389_43899_20201023_ELISA_PAIR_20110422_360.jpg "Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389")
![Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389](/upload/website/prouct_img/normal/GTX635389/GTX635389_43899_20201022_ELISA_PAIR_20110422_905.jpg "Anti-CA19-9 antibody [GT933] used in ELISA (ELISA). GTX635389")
![Anti-CA19-9 antibody [GT933] used in Flow cytometry (FACS). GTX635389](/upload/website/prouct_img/normal/GTX635389/GTX635389_43899_20200617_FACS_20062300_389.jpg "Anti-CA19-9 antibody [GT933] used in Flow cytometry (FACS). GTX635389")
![Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389](/upload/website/prouct_img/normal/GTX635389/GTX635389_43899_20210428_IHC-P_competitor_2_21050419_189.jpg "Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389")
![Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389](/upload/website/prouct_img/normal/GTX635389/GTX635389_43899_20210428_IHC-P_competitor_3_21050419_192.jpg "Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389")
![Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389](/upload/website/prouct_img/normal/GTX635389/GTX635389_43899_20210428_IHC-P_competitor_1_21050419_587.jpg "Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389")
![Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667](/upload/website/prouct_img/small/GTX16667/GTX16667_20160307_IHC-P_18121410_764.jpg "Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667")
![Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362](/upload/website/prouct_img/small/GTX635362/GTX635362_20200217_IHC-P_20021701_326.jpg "Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362")
