GeneTex

  • Country / Location Selection

United States (US)

CACNA2D1 antibody [20A]

Anti-CACNA2D1 antibody [20A] used in IHC (Paraffin sections) (IHC-P). GTX22864
Anti-CACNA2D1 antibody [20A] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22864
Anti-CACNA2D1 antibody [20A] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22864
Anti-CACNA2D1 antibody [20A] used in Flow cytometry (FACS). GTX22864
Anti-CACNA2D1 antibody [20A] used in Flow cytometry (FACS). GTX22864
Anti-CACNA2D1 antibody [20A] used in Western Blot (WB). GTX22864
Anti-CACNA2D1 antibody [20A] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22864
Anti-CACNA2D1 antibody [20A] used in IHC (Paraffin sections) (IHC-P). GTX22864
Anti-CACNA2D1 antibody [20A] used in Flow cytometry (FACS). GTX22864

Cat. No. GTX22864

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 20A

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Mouse, Rat, Rabbit, Guinea pig, Pig, Fish
Package
100 μl ($399)
See all CACNA2D1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:250
IHC-P 1:500
IHC-Fr 1:500
FACS 1:100-1:200
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rabbit

Immunogen

Purified rabbit dihydropyridine receptor.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium voltage-gated channel auxiliary subunit alpha2delta 1

Cellular Localization

Membrane

Database

Research Area

DATA IMAGES

Anti-CACNA2D1 antibody [20A] used in IHC (Paraffin sections) (IHC-P). GTX22864

GTX22864 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX22864 CACNA2D1 antibody [20A].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-CACNA2D1 antibody [20A] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22864

GTX22864 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22864 CACNA2D1 antibody [20A]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-CACNA2D1 antibody [20A] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22864

GTX22864 ICC/IF Image

ICC/IF analysis of PC-12 cells using GTX22864 CACNA2D1 antibody [20A]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-CACNA2D1 antibody [20A] used in Flow cytometry (FACS). GTX22864

GTX22864 FACS Image

FACS analysis of SH-SY5Y cells using GTX22864 CACNA2D1 antibody [20A] compared to an isotype control (blue).
Dilution : 1:100 for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-CACNA2D1 antibody [20A] used in Flow cytometry (FACS). GTX22864

GTX22864 FACS Image

FACS analysis of Neuro-2a cells using GTX22864 CACNA2D1 antibody [20A] compared to an isotype control (blue).
Dilution : 1:100 for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-CACNA2D1 antibody [20A] used in Western Blot (WB). GTX22864

GTX22864 WB Image

WB analysis of 25 ug of U87-MG (lane 1) and mouse brain (lane 2) cell lysates using GTX22864 CACNA2D1 antibody [20A].
Dilution : 1:1000

Anti-CACNA2D1 antibody [20A] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22864

GTX22864 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22864 CACNA2D1 antibody [20A]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-CACNA2D1 antibody [20A] used in IHC (Paraffin sections) (IHC-P). GTX22864

GTX22864 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX22864 CACNA2D1 antibody [20A].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-CACNA2D1 antibody [20A] used in Flow cytometry (FACS). GTX22864

GTX22864 FACS Image

FACS analysis of C6 cells using GTX22864 CACNA2D1 antibody [20A] compared to an isotype control (blue).
Dilution : 1:100 for 60 min at room temperature
Fixation : 2% paraformaldehyde

REFERENCE

Application Reference

Okada T et al. Sci Adv 2021; 7 (12) Pain induces stable, active microcircuits in the somatosensory cortex that provide a therapeutic target.
Application : FACS
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for CACNA2D1 antibody [20A] (GTX22864). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399