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CAD antibody [C2C3], C-term

Anti-CAD antibody [C2C3], C-term used in Western Blot (WB). GTX101401
Anti-CAD antibody [C2C3], C-term used in Western Blot (WB). GTX101401
Anti-CAD antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101401

Cat. No. GTX101401

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CAD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

243 kDa. ( Note )

Positive Control

293T , C2C12

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CAD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240541

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase , CDG1Z , EIEE50 , GATD4

Cellular Localization

Cytoplasm

Background

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CAD antibody [C2C3], C-term used in Western Blot (WB). GTX101401

GTX101401 WB Image

Sample (30μg whole cell lysate)
A:293T whole cell lysate
5% SDS PAGE
GTX101401 diluted at 1:1500

Anti-CAD antibody [C2C3], C-term used in Western Blot (WB). GTX101401

GTX101401 WB Image

CAD antibody [C2C3], C-term detects CAD protein by Western blot analysis.
A. 30 μg C2C12 whole cell lysate/extract
5 % SDS-PAGE
CAD antibody [C2C3], C-term (GTX101401) dilution: 1:1000

Anti-CAD antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101401

GTX101401 IHC-P Image

Immunohistochemical analysis of human salivary gland cancer, using CAD(GTX101401) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169