GeneTex
United States (US)

CAD antibody [C2C3], C-term

GTX101401_39694_IHC_19011506_588.jpg
GTX101401_WB_19011506_225.jpg
GTX101401_39694_WB_M_19011506_484.jpg

Cat No. GTX101401

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

243 kDa. ( Note )

Positive Control

293T , C2C12

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CAD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CAD antibody, CAD trifunctional protein antibody, multifunctional protein CAD antibody, CAD protein antibody, carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase antibody

Cellular Localization

Cytoplasm

Background

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101401_39694_IHC_19011506_588.jpg

GTX101401 IHC-P Image

Immunohistochemical analysis of human salivary gland cancer, using CAD(GTX101401) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101401_WB_19011506_225.jpg

GTX101401 WB Image

Sample (30μg whole cell lysate)
A:293T whole cell lysate
5% SDS PAGE
GTX101401 diluted at 1:1500

GTX101401_39694_WB_M_19011506_484.jpg

GTX101401 WB Image

CAD antibody [C2C3], C-term detects CAD protein by Western blot analysis.
A. 30 µg C2C12 whole cell lysate/extract
5 % SDS-PAGE
CAD antibody [C2C3], C-term (GTX101401) dilution: 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169