GeneTex
United States (US)

CAD antibody

CAD-antibody_ICCIF_GTX28406-1_18121409_837.jpg
CAD-antibody_WB_GTX28406-1_18121409_982.jpg

Cat No. GTX28406

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

Western blot at 1:500 to 1:1000 dilution.

Positive Control

K562 or Jurkat whole cell lysate A 40 kDa band can be detected.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Peptide corresponding to amino acids 147 to 164 of murineCAD (1). The sequence differs from human DFF40 by twoamino acids (3,4)

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Carbamoyl-Phosphate Synthetase 2, Aspartate Transcarbamylase, And Dihydroorotase,2410008J01Rik,Au018859,Cpad,Cad

Cellular Localization

Cytoplasmic and Nuclear.

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (1,2). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively, (3-5). DFF45/ICAD is the inhibitory protein of DFF40/CAD (1,2,6) and forms complex with DFF40/CAD. Upon cleavage ofDFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.

Research Area

DATA IMAGES
CAD-antibody_ICCIF_GTX28406-1_18121409_837.jpg

GTX28406 ICC/IF Image

Immunocytochemical staining of DFF40 in Jurkat cells with CAD antibody (GTX28406) at 5μg/ml.

CAD-antibody_WB_GTX28406-1_18121409_982.jpg

GTX28406 WB Image

Western blot analysis of DFF40/CAD in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF40/CAD antibody (GTX28406) at 1μg/ml.

Package List Price ($)
$ 349