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CALM antibody

Anti-CALM antibody used in Western Blot (WB). GTX119084
Anti-CALM antibody used in IHC (Paraffin sections) (IHC-P). GTX119084

Cat. No. GTX119084

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CALM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CALM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166421

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphatidylinositol binding clathrin assembly protein , CALM , CLTH , LAP

Cellular Localization

Membrane , clathrin-coated pit , Golgi apparatus , Cytoplasmic vesicle , clathrin-coated vesicle

Background

Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.

Database

Research Area

DATA IMAGES

Anti-CALM antibody used in Western Blot (WB). GTX119084

GTX119084 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX119084 diluted at 1:2000

Anti-CALM antibody used in IHC (Paraffin sections) (IHC-P). GTX119084

GTX119084 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human Renal Tubule, using CALM(GTX119084) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169