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CALM antibody

Anti-CALM antibody used in IHC (Paraffin sections) (IHC-P). GTX85213
Anti-CALM antibody used in Western Blot (WB). GTX85213

Cat. No. GTX85213

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all CALM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , EL4 Cell Lysate(GTX27183)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PICALM antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human PICALM.The immunogen is located within the first 50 amino acids of PICALM.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphatidylinositol binding clathrin assembly protein , CALM , CLTH , LAP

Cellular Localization

Cell membrane,Golgi apparatus,Nucleus

Background

PICALM, also known as the clathrin assembly lymphoid myeloid leukemia (CALM) protein, co-localizes with clathrin at clathrin-coated areas of the plasma membrane and in the Golgi area, and is thought to be an important component of the coated pit internalization machinery. Fusion proteins involving PICALM have been implicated in acute myeloid and T-cell acute lymphoblastic leukemia. PICALM is also expressed in neurons and is present at the synapse where it facilitates the endocytosis of the synaptic vesicle protein VAMP2. Recent studies have identified variants of PICALM associated with Alzheimer's disease and have suggested this protein may play a role in the endocytosis and recycling of the amyloid protein precursor (APP).

Database

Research Area

DATA IMAGES

Anti-CALM antibody used in IHC (Paraffin sections) (IHC-P). GTX85213

GTX85213 IHC-P Image

IHC-P analysis of human brain tissue using GTX85213 CALM antibody.
Working concentration : 20 μg/ml

Anti-CALM antibody used in Western Blot (WB). GTX85213

GTX85213 WB Image

WB analysis of EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX85213 CALM antibody.
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399