GeneTex
United States (US)

CALML3 antibody [N1C3]

GTX114954_40359_IFA_18121410_687.jpg
GTX114954_40359_WB_18121410_252.jpg
GTX114954_40359_20160616_WB_M_liver_18121410_652.jpg
GTX114954_40359_IHC_18121410_616.jpg

Cat No. GTX114954

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

A431 , mouse liver

Predict Reactivity

Rat, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CALML3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CLP antibody, CALML3 antibody, calmodulin-like protein 3 antibody, calmodulin-related protein NB-1 antibody, caM-like protein antibody, calmodulin-like 3 antibody

Background

May be similar to that of authentic calmodulin and may actually compete with calmodulin by binding, with different affinities, to cellular substrates.
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Database

Research Area

DATA IMAGES
GTX114954_40359_IFA_18121410_687.jpg

GTX114954 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using CALML3(GTX114954) antibody at 1:200 dilution.

GTX114954_40359_WB_18121410_252.jpg

GTX114954 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
15% SDS PAGE
GTX114954 diluted at 1:1000

GTX114954_40359_20160616_WB_M_liver_18121410_652.jpg

GTX114954 WB Image

Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with CALML3 antibody [N1C3] (GTX114954) diluted at 1:1000.

GTX114954_40359_IHC_18121410_616.jpg

GTX114954 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using CALML3(GTX114954) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf