Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with CALML5 antibody [N1C3] (GTX119159) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human hepatoma, using CALML5(GTX119159) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min