GeneTex

  • Country / Location Selection

United States (US)

CAPZA1 antibody [2G4]

Overexpression
Anti-CAPZA1 antibody [2G4] used in Flow cytometry (FACS). GTX84737
Anti-CAPZA1 antibody [2G4] used in Western Blot (WB). GTX84737
Anti-CAPZA1 antibody [2G4] used in IHC (Paraffin sections) (IHC-P). GTX84737
Anti-CAPZA1 antibody [2G4] used in Western Blot (WB). GTX84737
Anti-CAPZA1 antibody [2G4] used in IHC (Paraffin sections) (IHC-P). GTX84737
Anti-CAPZA1 antibody [2G4] used in IHC (Paraffin sections) (IHC-P). GTX84737

Cat. No. GTX84737

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2G4

Isotype

 IgG1

Application

 WB, IHC-P, FACS

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($519)
See all CAPZA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:500
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CAPZA1(NP_006126) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

capping actin protein of muscle Z-line subunit alpha 1 , CAPPA1 , CAPZ , CAZ1

Cellular Localization

Cytoplasm, cytoskeleton

Background

F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.

Database

Research Area

DATA IMAGES

Anti-CAPZA1 antibody [2G4] used in Flow cytometry (FACS). GTX84737

GTX84737 FACS Image

FACS analysis of Jurkat cells using GTX84737 CAPZA1 antibody [2G4].
Red : Primary antibody
Blue : Negative control antibody
Dilution : 1:100

Anti-CAPZA1 antibody [2G4] used in Western Blot (WB). GTX84737

GTX84737 WB Image

WB analysis of various cell lines using GTX84737 CAPZA1 antibody [2G4].
Loading : 35 ug per lane
Dilution : 1:200

Anti-CAPZA1 antibody [2G4] used in IHC (Paraffin sections) (IHC-P). GTX84737

GTX84737 IHC-P Image

IHC-P analysis of prostate tissue using GTX84737 CAPZA1 antibody [2G4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-CAPZA1 antibody [2G4] used in Western Blot (WB). GTX84737

GTX84737 WB Image

Overexpression

WB analysis of HEK293T cells transfected with CAPZA1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84737 CAPZA1 antibody [2G4].
Loading : 5 ug per lane

Anti-CAPZA1 antibody [2G4] used in IHC (Paraffin sections) (IHC-P). GTX84737

GTX84737 IHC-P Image

IHC-P analysis of liver tissue using GTX84737 CAPZA1 antibody [2G4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-CAPZA1 antibody [2G4] used in IHC (Paraffin sections) (IHC-P). GTX84737

GTX84737 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84737 CAPZA1 antibody [2G4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

There are currently no references for CAPZA1 antibody [2G4] (GTX84737). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for CAPZA1 antibody [2G4] (GTX84737). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519