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CAPZB antibody

Anti-CAPZB antibody used in Western Blot (WB). GTX101686
Anti-CAPZB antibody used in IHC (Paraffin sections) (IHC-P). GTX101686
Anti-CAPZB antibody used in Western Blot (WB). GTX101686

Cat No. GTX101686

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

A431 , H1299 , HepG2 , mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CAPZB. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

capping actin protein of muscle Z-line subunit beta , CAPB , CAPPB , CAPZ

Cellular Localization

Cytoplasm , cytoskeleton

Background

CAPZB is a member of the F-actin capping protein family. This gene encodes the beta subunit of the barbed-end actin binding protein. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CAPZB antibody used in Western Blot (WB). GTX101686

GTX101686 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
GTX101686 diluted at 1:1000

Anti-CAPZB antibody used in IHC (Paraffin sections) (IHC-P). GTX101686

GTX101686 IHC-P Image

Immunohistochemical analysis of paraffin-embedded 59T xenograft, using CapZ beta(GTX101686) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CAPZB antibody used in Western Blot (WB). GTX101686

GTX101686 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX101686 diluted at 1:1000

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf