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CARD10 antibody [HL2260]

Recombinant antibody KO/KD Orthogonal
Anti-CARD10 antibody [HL2260] used in Western Blot (WB). GTX638312
Anti-CARD10 antibody [HL2260] used in Western Blot (WB). GTX638312
Anti-CARD10 antibody [HL2260] used in IHC (Paraffin sections) (IHC-P). GTX638312
Anti-CARD10 antibody [HL2260] used in Western Blot (WB). GTX638312

Cat. No. GTX638312

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2260

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CARD10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human CARD10.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase recruitment domain family member 10 , BIMP1 , CARMA3

Cellular Localization

Cytoplasm

Background

The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-CARD10 antibody [HL2260] used in Western Blot (WB). GTX638312

GTX638312 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [HL2260] (GTX638312) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CARD10 antibody [HL2260] used in Western Blot (WB). GTX638312

GTX638312 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [HL2260] (GTX638312) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CARD10 antibody [HL2260] used in IHC (Paraffin sections) (IHC-P). GTX638312

GTX638312 IHC-P Image

CARD10 antibody [HL2260] detects CARD10 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
CARD10 stained by CARD10 antibody [HL2260] (GTX638312) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CARD10 antibody [HL2260] used in Western Blot (WB). GTX638312

GTX638312 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [HL2260] (GTX638312) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations