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CARD10 antibody [N2C1], Internal

KO/KD Orthogonal
Anti-CARD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111222
Anti-CARD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111222
Anti-CARD10 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111222
Anti-CARD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111222
Anti-CARD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111222
Anti-CARD10 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111222
Anti-CARD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111222

Cat. No. GTX111222

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CARD10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

Positive Control

mouse heart

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CARD10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949831

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase recruitment domain family member 10 , BIMP1 , CARMA3

Cellular Localization

Cytoplasm

Background

The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-CARD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111222

GTX111222 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CARD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111222

GTX111222 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CARD10 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111222

GTX111222 IHC-P Image

CARD10 antibody [N2C1], Internal detects CARD10 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CARD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111222

GTX111222 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CARD10 antibody (GTX111222) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CARD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111222

GTX111222 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CARD10 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111222

GTX111222 IHC-P Image

CARD10 antibody [N2C1], Internal detects CARD10 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CARD10 stained by CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CARD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111222

GTX111222 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

McAuley JR et al. Oncogene 2019; (Epub) MALT1 is a critical mediator of PAR1-driven NF-庥B activation and metastasis in multiple tumor types.
Application : WB
Reactivity : Human
Kahle JJ et al. Hum Mol Genet 2011; 20 (3) : 510-27 Comparison of an expanded ataxia interactome with patient medical records reveals a relationship between macular degeneration and ataxia.
Application : IHC-Fr
Reactivity : Mouse
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169