APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
BCL-1 , Raji , K562 , NCI-H929
Predict Reactivity
Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.02 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human CARD11. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2036434
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
caspase recruitment domain family member 11 , BENTA , BIMP3 , CARMA1 , IMD11 , IMD11A , PPBL
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-CARD11 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113328](/templates/default/js/lightbox/images/loading.gif "Anti-CARD11 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113328")
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GTX113328 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using CARD11(GTX113328) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113328 WB Image
CARD11 antibody [N1N2], N-term detects CARD11 protein by western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
5% SDS-PAGE
CARD11 antibody [N1N2], N-term (GTX113328) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113328 WB Image
CARD11 antibody [N1N2], N-term detects CARD11 protein by western blot analysis.
A. 30 μg Raji whole cell lysate/extract
B. 30 μg K562 whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
5% SDS-PAGE
CARD11 antibody [N1N2], N-term (GTX113328) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for CARD11 antibody [N1N2], N-term (GTX113328). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CARD11 antibody [N1N2], N-term (GTX113328). Be the first to share your experience with this product.
![Anti-CARD11 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113328](/upload/website/prouct_img/small/GTX113328/GTX113328_40128_IHC_w_23060501_498.webp "Anti-CARD11 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113328")
![Anti-CARD11 antibody [N1N2], N-term used in Western Blot (WB). GTX113328](/upload/website/prouct_img/small/GTX113328/GTX113328_40128_WB_M_w_23060501_267.webp "Anti-CARD11 antibody [N1N2], N-term used in Western Blot (WB). GTX113328")
![Anti-CARD11 antibody [N1N2], N-term used in Western Blot (WB). GTX113328](/upload/website/prouct_img/small/GTX113328/GTX113328_40128_WB_w_23060501_329.webp "Anti-CARD11 antibody [N1N2], N-term used in Western Blot (WB). GTX113328")
![Anti-CARD11 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113328](/upload/website/prouct_img/normal/GTX113328/GTX113328_40128_IHC_w_23060501_498.webp "Anti-CARD11 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113328")
![Anti-CARD11 antibody [N1N2], N-term used in Western Blot (WB). GTX113328](/upload/website/prouct_img/normal/GTX113328/GTX113328_40128_WB_M_w_23060501_267.webp "Anti-CARD11 antibody [N1N2], N-term used in Western Blot (WB). GTX113328")
![Anti-CARD11 antibody [N1N2], N-term used in Western Blot (WB). GTX113328](/upload/website/prouct_img/normal/GTX113328/GTX113328_40128_WB_w_23060501_329.webp "Anti-CARD11 antibody [N1N2], N-term used in Western Blot (WB). GTX113328")