CARD12 antibody [GT1387]

Cat. No. GTX631645

Host	Mouse
Clonality	Monoclonal
Clone Name	GT1387
Isotype	IgG2a
Application	WB
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

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Datasheet
Document

View product citations for antibody GTX631645 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000

Not tested in other applications.

Calculated MW

116 kDa. ( Note )

Positive Control

human NLRC4-transfected 293T cells

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CARD12. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888250

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NLR family CARD domain containing 4 , AIFEC , CARD12 , CLAN , CLAN1 , CLANA , CLANB , CLANC , CLAND , CLR2.1 , FCAS4 , IPAF

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Database

Gene ID: 58484 NLRC4
UniProt: Q9NPP4 NLRC4

Research Area

DATA IMAGES

GTX631645 WB Image

CARD12 antibody detects CARD12 protein by western blot analysis. Non-transfected (-) and CARD12 -transfected (+, ) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD12 antibody (GTX631645) diluted by 1:5000.

REFERENCE

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REVIEW

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SDS
PBS.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations