GeneTex
United States (US)

CARD12 antibody [N1], N-term

Overexpression
GTX102366_40835_WB_19011506_813.jpg
GTX102366_40835_WB_B_19011506_800.jpg

Cat No. GTX102366

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

Positive Control

MCF-7 , human NLRC4-transfected 293T

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CARD12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nlr Family Card Domain Containing 4,Aifec,Card12,Clan,Clan1,Clana,Clanb,Clanc,Cland,Clr2.1,Fcas4,Ipaf,Nlrc4

Cellular Localization

Cytoplasm

Background

In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1 (MIM 602233), NOD1/CARD4 (MIM 605980), and NOD2/CARD15 (MIM 605956). These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX102366_40835_WB_19011506_813.jpg

GTX102366 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
5% SDS PAGE
GTX102366 diluted at 1:1000

GTX102366_40835_WB_B_19011506_800.jpg

GTX102366 WB Image

Overexpression

CARD12 antibody detects CARD12 protein by western blot analysis. Non-transfected (-) and CARD12 -transfected (+, ) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD12 antibody (GTX102366) diluted by 1:5000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169