*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
MCF-7 , human NLRC4-transfected 293T
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the N-terminus region of human CARD12. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
NLR family CARD domain containing 4 , AIFEC , CARD12 , CLAN , CLAN1 , CLANA , CLANB , CLANC , CLAND , CLR2.1 , FCAS4 , IPAF
In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1 (MIM 602233), NOD1/CARD4 (MIM 605980), and NOD2/CARD15 (MIM 605956). These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.[supplied by OMIM]
GTX102366 WB Image
Sample (30 ug of whole cell lysate)
5% SDS PAGE
GTX102366 diluted at 1:1000
GTX102366 WB Image
CARD12 antibody detects CARD12 protein by western blot analysis. Non-transfected (-) and CARD12 -transfected (+, ) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD12 antibody (GTX102366) diluted by 1:5000.