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CARD12 antibody

Anti-CARD12 antibody used in Western Blot (WB). GTX04516

Cat. No. GTX04516

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μg ($469)
See all CARD12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/mL
ICC/IF 5 μg/mL
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

0.05 mg Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CARD12 (838-874aa KILAQNLHNLVKLSILDLSENYLEKDGNEALHELIDR)

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AIFEC,CARD12,CLAN,CLAN1,CLANA,CLANB,CLANC,CLAND,CLR2.1,FCAS4,IPAF,NLR family CARD domain containing 4,NLRC4

Cellular Localization

Cytoplasm,Cytosol

Background

This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES

Anti-CARD12 antibody used in Western Blot (WB). GTX04516

GTX04516 WB Image

WB analysis of various lysates using GTX04516 CARD12 antibody.
Lane 1 : 22RV1 whole cell lysate
Lane 2 : RH35 whole cell lysate
Lane 3 : NIH-3T3 whole cell lysate
Loading : 40 μg
Dilution : 0.5 μg/mL

REFERENCE

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REVIEW

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Package List Price ($)
$ 469