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CARD14 antibody

Anti-CARD14 antibody used in Western Blot (WB). GTX55231
Anti-CARD14 antibody used in IHC (Paraffin sections) (IHC-P). GTX55231
Anti-CARD14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55231

Cat. No. GTX55231

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all CARD14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of CARD14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase recruitment domain family member 14 , BIMP2 , CARMA2 , PRP , PSORS2 , PSS1

Background

This gene encodes a caspase recruitment domain-containing protein that is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. Members of this protein family are scaffold proteins that are involved in a diverse array of cellular processes including cellular adhesion, signal transduction and cell polarity control. This protein has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES

Anti-CARD14 antibody used in Western Blot (WB). GTX55231

GTX55231 WB Image

WB analysis of HeLa (A) whole cell lysates using GTX55231 CARD14 antibody.

Anti-CARD14 antibody used in IHC (Paraffin sections) (IHC-P). GTX55231

GTX55231 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX55231 CARD14 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-CARD14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55231

GTX55231 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX55231 CARD14 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399