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CARD6 antibody [C1C3]

Anti-CARD6 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111277
Anti-CARD6 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111277
Anti-CARD6 antibody [C1C3] used in Western Blot (WB). GTX111277

Cat. No. GTX111277

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CARD6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

Positive Control

A549

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CARD6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722597

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase recruitment domain family member 6 , CINCIN1

Background

This gene encodes a protein that contains a caspase recruitment domain (CARD), an antiparallel six-helical bundle that mediates homotypic protein-protein interactions. The encoded protein is a microtubule-associated protein that has been shown to interact with receptor-interacting protein kinases and positively modulate signal transduction pathways converging on activation of the inducible transcription factor NF-kB. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CARD6 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111277

GTX111277 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using CARD6(GTX111277) antibody at 1:500 dilution.

Anti-CARD6 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111277

GTX111277 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using CARD6(GTX111277) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CARD6 antibody [C1C3] used in Western Blot (WB). GTX111277

GTX111277 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX111277 diluted at 1:5000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169