GeneTex
United States (US)

CARD6 antibody [N1N2], N-term

GTX106888_WB_19011506_961.jpg
GTX106888_39981_IFA_19011506_314.jpg

Cat No. GTX106888

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

Positive Control

HeLa , HepG2
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CARD6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CINCIN1 antibody, CARD6 antibody, caspase recruitment domain-containing protein 6 antibody, caspase recruitment domain protein 6 antibody, CARD-containing inhibitor of Nod1 and Cardiak-induced NF-kB activation antibody, "caspase recruitment domain family, member 6 antibody"

Background

This gene encodes a protein that contains a caspase recruitment domain (CARD), an antiparallel six-helical bundle that mediates homotypic protein-protein interactions. The encoded protein is a microtubule-associated protein that has been shown to interact with receptor-interacting protein kinases and positively modulate signal transduction pathways converging on activation of the inducible transcription factor NF-kB. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106888_WB_19011506_961.jpg

GTX106888 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX106888 diluted at 1:5000

GTX106888_39981_IFA_19011506_314.jpg

GTX106888 ICC/IF Image

CARD6 antibody [N1N2], N-term detects CARD6 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CARD6 protein stained by CARD6 antibody [N1N2], N-term (GTX106888) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169