GeneTex
United States (US)

CARD8 antibody [N3C3]

GTX102395_IFA_19011506_474.jpg
GTX102395_WB_19011506_363.jpg

Cat No. GTX102395

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T , A431 , HepG2
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CARD8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CARDINAL antibody, DACAR antibody, DAKAR antibody, DKFZp779L0366 antibody, FLJ18119 antibody, FLJ18121 antibody, KIAA0955 antibody, MGC57162 antibody, NDPP antibody, NDPP1 antibody, TUCAN antibody, CARD8 antibody, apoptotic protein NDPP1 antibody, caspase recruitment domain-containing protein 8 antibody, CARD inhibitor of NF-kappaB-activating ligands antibody, tumor up-regulated CARD-containing antagonist of CASP9 antibody, tumor up-regulated CARD-containing antagonist of caspase nine antibody, "caspase recruitment domain family, member 8 antibody"

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene, but their biological validity has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102395_IFA_19011506_474.jpg

GTX102395 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CARD8(GTX102395) antibody at 1:200 dilution.

GTX102395_WB_19011506_363.jpg

GTX102395 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
B:Hep G2(GTX27900)
10% SDS PAGE
GTX102395 diluted at 1:500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169