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CARD8 antibody

Anti-CARD8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04354
Anti-CARD8 antibody used in Western Blot (WB). GTX04354
Anti-CARD8 antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX04354

Cat. No. GTX04354

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, IHC-P (cell pellet)

Reactivity

 Human
Package
100 μg ($429)
See all CARD8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/mL
ICC/IF 20 μg/mL
ELISA Assay dependent
IHC-P (cell pellet) 10 μg/mL
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CARD8 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARD8. The immunogen is located within the last 50 amino acids of CARD8.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase recruitment domain family member 8 , CARDINAL , DACAR , DAKAR , NDPP , NDPP1 , TUCAN

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-CARD8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04354

GTX04354 ICC/IF Image

ICC/IF analysis of K562 cells using GTX04354 CARD8 antibody.
Dilution : 20 μg/mL

Anti-CARD8 antibody used in Western Blot (WB). GTX04354

GTX04354 WB Image

WB analysis of human lung tissue lysates using GTX04354 CARD8 antibody.
Dilution :
Lane A : 1 μg/mL
Lane B : 2 μg/mL

Anti-CARD8 antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX04354

GTX04354 IHC-P (cell pellet) Image

IHC-P (cell pellet) analysis of K562 cells using GTX04354 CARD8 antibody.
Dilution : 10 μg/mL

REFERENCE

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REVIEW

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Package List Price ($)
$ 429