GeneTex
United States (US)

CARD9 antibody

CARD-9-antibody_WB_GTX29649-1_18121409_369.jpg
CARD-9-antibody_ICCIF_GTX29649-1_18121409_175.jpg

Cat No. GTX29649

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

For WB: Use at a concentration of 0.5-1.0 μg/ml. A 59 kD band is detected. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

62 kDa. ( Note )

Positive Control

PC-3 cell lysate
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4, containing 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide (DRENTTGSDNTDTEGS) corresponding to amino acids 521 to 536 of human CARD9 (1). The sequence is different from that of rat origin by two amino acids (1).

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD9, which interacts with the CARD activation domain of BCL-10 (1). CARD9 associates with BCL-10 and forms a complex within cells. CARD9 induces apoptosis and activates NF-kB. CARD9 is an upstream activator of BCL-10 and NF-kB signaling.

Database

Research Area

DATA IMAGES
CARD-9-antibody_WB_GTX29649-1_18121409_369.jpg

GTX29649 WB Image

Western blot analysis of CARD9 expression in human MDA-MB-361 (A) and PC-3 (B) cell lysate with Anti-CARD9 (Cat. No. GTX29649) at 2.5 μg/mL.

CARD-9-antibody_ICCIF_GTX29649-1_18121409_175.jpg

GTX29649 ICC/IF Image

Immunocytochemical staining of CARD9 in K562 cells with CARD9 antibody (GTX29649) at 10μg/ml.

Package List Price ($)
$ 349