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CARD9 antibody

Anti-CARD9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29649
Anti-CARD9 antibody used in Western Blot (WB). GTX29649

Cat. No. GTX29649

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μg ($479)
See all CARD9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2-3μg/ml
ICC/IF 10μg/ml
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to aa 521-536 of human CARD9 (accession no. AF31187). This sequence differs from that of rat CARD9 by two amino acids.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CANDF2 , CARD9 , caspase recruitment domain family member 9 , hCARD9

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the CARD protein family, which is defined by the presence of a characteristic caspase-associated recruitment domain (CARD). CARD is a protein interaction domain known to participate in activation or suppression of CARD containing members of the caspase family, and thus plays an important regulatory role in cell apoptosis. This protein was identified by its selective association with the CARD domain of BCL10, a postive regulator of apoptosis and NF-kappaB activation, and is thought to function as a molecular scaffold for the assembly of a BCL10 signaling complex that activates NF-kappaB. Several alternatively spliced transcript variants have been observed, but their full-length nature is not clearly defined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CARD9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29649

GTX29649 ICC/IF Image

ICC/IF analysis of K562 cells using GTX29649 CARD9 antibody.
Dilution : 10μg/ml

Anti-CARD9 antibody used in Western Blot (WB). GTX29649

GTX29649 WB Image

WB analysis of MDAMB-361 (A) and PC-3 (B) cell lysates using GTX29649 CARD9 antibody.
Dilution : 2.5μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 479