GeneTex
United States (US)

CARM1 antibody

GTX31871_385_ICC-IF_20180221_18121410_673.jpg
GTX31871_3019_WB_20180221_18121410_487.jpg

Cat No. GTX31871

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

Jurkat Cell Slide(GTX25533) , Jurkat Cell Lysate(GTX27899)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Rabbit polyclonal CARM1 antibody was raised against a 19 amino acid peptide near the amino terminus of human CARM1.The immunogen is located within amino acids 470 - 520 of CARM1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Coactivator Associated Arginine Methyltransferase 1,Prmt4,Carm1

Background

Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 [PubMed 11724789]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES
GTX31871_385_ICC-IF_20180221_18121410_673.jpg

GTX31871 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX31871 CARM1 antibody.
Working concentration : 5 μg/ml

GTX31871_3019_WB_20180221_18121410_487.jpg

GTX31871 WB Image

WB analysis of Jurkat cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31871 CARM1 antibody.
Working concentration : 1 μg/ml