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CARM1 antibody

Anti-CARM1 antibody used in Western Blot (WB). GTX31871
Anti-CARM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31871

Cat. No. GTX31871

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all CARM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

Jurkat Cell Slide(GTX25533) , Jurkat Cell Lysate(GTX27899)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Rabbit polyclonal CARM1 antibody was raised against a 19 amino acid peptide near the amino terminus of human CARM1.The immunogen is located within amino acids 470 - 520 of CARM1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coactivator associated arginine methyltransferase 1 , PRMT4

Cellular Localization

Nucleus,Cytoplasm

Background

Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 [PubMed 11724789]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-CARM1 antibody used in Western Blot (WB). GTX31871

GTX31871 WB Image

WB analysis of Jurkat cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31871 CARM1 antibody.
Working concentration : 1 μg/ml

Anti-CARM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31871

GTX31871 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX31871 CARM1 antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399