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CARM1 antibody

Orthogonal
Anti-CARM1 antibody used in Western Blot (WB). GTX78920

Cat. No. GTX78920

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all CARM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized non-phosphopeptide derived from human CARM1 around the phosphorylation site of serine 228 (V-K-Sp-N-N).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_11162682

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CARM1,PRMT4,coactivator associated arginine methyltransferase 1

Cellular Localization

Nucleus,Cytoplasm

Background

This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9. [provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-CARM1 antibody used in Western Blot (WB). GTX78920

GTX78920 WB Image

Orthogonal

WB analysis of A431 cells untreated or treated with EGF (200ng/ml, 5min) lysate using GTX78920 CARM1 antibody (Lane 1 and 2) and CARM1 (Phospho-Ser228) antibody (Lane 3 and 4).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399