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CARS antibody

Anti-CARS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32489
Anti-CARS antibody used in IHC (Paraffin sections) (IHC-P). GTX32489
Anti-CARS antibody used in Western Blot (WB). GTX32489

Cat. No. GTX32489

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all CARS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 469-748 of human CARS (NP_001742.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cysteinyl-tRNA synthetase , CARS1 , CYSRS , MGC:11246

Cellular Localization

Cytoplasm

Background

This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2010]

Database

DATA IMAGES

Anti-CARS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32489

GTX32489 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32489 CARS antibody.
Blue : DAPI

Anti-CARS antibody used in IHC (Paraffin sections) (IHC-P). GTX32489

GTX32489 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX32489 CARS antibody.
Dilution : 1:100

Anti-CARS antibody used in Western Blot (WB). GTX32489

GTX32489 WB Image

WB analysis of various sample lysates using GTX32489 CARS antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399