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CASK antibody

Anti-CASK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133275
Anti-CASK antibody used in IHC (Paraffin sections) (IHC-P). GTX133275
Anti-CASK antibody used in IHC (Paraffin sections) (IHC-P). GTX133275
Anti-CASK antibody used in Western Blot (WB). GTX133275

Cat No. GTX133275

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse CASK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

calcium/calmodulin-dependent serine protein kinase (MAGUK family),DXPri1,DXRib1,LIN-2,Pals3,mLin-2

Cellular Localization

Nucleus , Cytoplasm , Cell membrane; Peripheral membrane protein , Nucleus , Cytoplasm , Cell membrane

Background

Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins, and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.

Database

Research Area

DATA IMAGES
Anti-CASK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133275

GTX133275 ICC/IF Image

CASK antibody detects CASK protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CASK protein stained by CASK antibody (GTX133275) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CASK antibody used in IHC (Paraffin sections) (IHC-P). GTX133275

GTX133275 IHC-P Image

CASK antibody detects CASK protein at cytoplasm in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
CASK antibody (GTX133275) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CASK antibody used in IHC (Paraffin sections) (IHC-P). GTX133275

GTX133275 IHC-P Image

CASK antibody detects CASK protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
CASK antibody (GTX133275) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CASK antibody used in Western Blot (WB). GTX133275

GTX133275 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CASK antibody (GTX133275) diluted at 1:5000.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169