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CASKIN2 antibody

Anti-CASKIN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31467
Anti-CASKIN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31467
Anti-CASKIN2 antibody used in Western Blot (WB). GTX31467

Cat No. GTX31467

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , HeLa Cell Lysate

Specificity/Sensitivity

CASKIN2 antibody is predicted to not cross-react with other CASKIN family members. Three isoforms of CASKIN2 are known to exist; this antibody will not detect the shortest isoform.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CASKIN2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human CASKIN2.The immunogen is located within amino acids 380 - 430 of CASKIN2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CASK interacting protein 2 , ANKS5B

Cellular Localization

Cytoplasm

Background

This gene encodes a large protein that contains six ankyrin repeats, as well as a Src homology 3 (SH3) domain and two sterile alpha motif (SAM) domains, which may be involved in protein-protein interactions. The C-terminal portion of this protein is proline-rich and contains a conserved region. A related protein interacts with calcium/calmodulin-dependent serine protein kinase (CASK). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Database

DATA IMAGES

Anti-CASKIN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31467

GTX31467 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31467 CASKIN2 antibody.
Working concentration : 2.5 μg/ml

Anti-CASKIN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31467

GTX31467 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31467 CASKIN2 antibody.
Working concentration : 20 μg/ml

Anti-CASKIN2 antibody used in Western Blot (WB). GTX31467

GTX31467 WB Image

WB analysis of HeLa cell lysate using GTX31467 CASKIN2 antibody.
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339