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CAT1 antibody

Anti-CAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103000
Anti-CAT1 antibody used in Western Blot (WB). GTX103000
Anti-CAT1 antibody used in Western Blot (WB). GTX103000

Cat No. GTX103000

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

293T , H1299 , Raji , rat muscle

Predict Reactivity

Mouse, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CAT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

carnitine O-acetyltransferase , CAT , CAT1 , NBIA8

Cellular Localization

Endoplasmic reticulum , Peroxisome , Mitochondrion inner membrane; Peripheral membrane protein; Matrix side , Endoplasmic reticulum , Peroxisome , Mitochondrion inner membrane

Background

Carnitine acetyltransferase (CRAT) is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments. Different subcellular localizations of the CRAT mRNAs are thought to result from alternative splicing of the CRAT gene suggested by the divergent sequences in the 5' region of peroxisomal and mitochondrial CRAT cDNAs and the location of an intron where the sequences diverge. The alternatively splicing of this gene results in three distinct isoforms, one of which contains an N-terminial mitochondrial transit peptide, and has been shown to be located in mitochondria. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-CAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103000

GTX103000 ICC/IF Image

Immunofluorescence analysis of methanol-fixed H1299, using CAT1(GTX103000) antibody at 1:500 dilution.

Anti-CAT1 antibody used in Western Blot (WB). GTX103000

GTX103000 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CAT1 antibody (GTX103000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CAT1 antibody used in Western Blot (WB). GTX103000

GTX103000 WB Image

Sample(30 µg of whole cell lysate)
A:Raji(GTX27908)
10% SDS PAGE
GTX103000 diluted at 1:1500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169