*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , H1299 , Raji , rat muscle
Mouse, Bovine, Pig, Rhesus Monkey(>80% identity)
1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.37 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human CAT1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
carnitine O-acetyltransferase , CAT , CAT1 , NBIA8
Endoplasmic reticulum , Peroxisome , Mitochondrion inner membrane; Peripheral membrane protein; Matrix side , Endoplasmic reticulum , Peroxisome , Mitochondrion inner membrane
Carnitine acetyltransferase (CRAT) is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments. Different subcellular localizations of the CRAT mRNAs are thought to result from alternative splicing of the CRAT gene suggested by the divergent sequences in the 5' region of peroxisomal and mitochondrial CRAT cDNAs and the location of an intron where the sequences diverge. The alternatively splicing of this gene results in three distinct isoforms, one of which contains an N-terminial mitochondrial transit peptide, and has been shown to be located in mitochondria. [provided by RefSeq]
GTX103000 ICC/IF Image
Immunofluorescence analysis of methanol-fixed H1299, using CAT1(GTX103000) antibody at 1:500 dilution.
GTX103000 WB Image
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CAT1 antibody (GTX103000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX103000 WB Image
Sample(30 µg of whole cell lysate)
10% SDS PAGE
GTX103000 diluted at 1:1500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.