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CBFb antibody - ChIP grade

Anti-CBFb antibody - ChIP grade used in ELISA (ELISA). GTX60333
Anti-CBFb antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60333
Anti-CBFb antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60333

Cat. No. GTX60333

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ELISA, ChIP assay

Reactivity

 Human
Package
50 μl ($399)
See all CBFb products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ELISA 1:500
ChIP assay 1-10 μl
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Human CBFb (core-binding factor, beta subunit) using two KLH-conjugated synthetic peptides containing sequences from the central region of the protein.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

core-binding factor subunit beta , PEBP2B

Cellular Localization

Nucleus

Background

The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CBFb antibody - ChIP grade used in ELISA (ELISA). GTX60333

GTX60333 ELISA Image

ELISA analysis of peptides used for immunization using GTX60333 CBFb antibody - ChIP grade.

Anti-CBFb antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60333

GTX60333 ChIP assay Image

ChIP analysis of SKNO-1 cells using GTX60333 CBFb antibody - ChIP grade. The IP'd DNA from 6 ChIP's was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions region surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.

Anti-CBFb antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60333

GTX60333 ChIP assay Image

ChIP analysis of sheared chromatin from 1.25x10⁶ SKNO-1 cells using GTX60333 CBFb antibody - ChIP grade. QPCR was performed using primers specific for the FUT7, OGG1, NFE2, and SPI1 genes. This figure shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.
Antibody amount : 4μl

REFERENCE

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REVIEW

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Package List Price ($)
$ 399