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CBFb antibody

Anti-CBFb antibody used in Western Blot (WB). GTX33066
Anti-CBFb antibody used in IHC (Paraffin sections) (IHC-P). GTX33066
Anti-CBFb antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33066
Anti-CBFb antibody used in IHC (Paraffin sections) (IHC-P). GTX33066
Anti-CBFb antibody used in IHC (Paraffin sections) (IHC-P). GTX33066
Anti-CBFb antibody used in IHC (Paraffin sections) (IHC-P). GTX33066

Cat No. GTX33066

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of human CBFB (NP_001746.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

core-binding factor subunit beta , PEBP2B

Cellular Localization

Nucleus

Background

The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-CBFb antibody used in Western Blot (WB). GTX33066

GTX33066 WB Image

WB analysis of various samples using GTX33066 CBFb antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CBFb antibody used in IHC (Paraffin sections) (IHC-P). GTX33066

GTX33066 IHC-P Image

IHC-P analysis of human kidney cancer tissue using GTX33066 CBFb antibody.
Dilution : 1:200

Anti-CBFb antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33066

GTX33066 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX33066 CBFb antibody.
Blue : DAPI

Anti-CBFb antibody used in IHC (Paraffin sections) (IHC-P). GTX33066

GTX33066 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX33066 CBFb antibody.
Dilution : 1:200

Anti-CBFb antibody used in IHC (Paraffin sections) (IHC-P). GTX33066

GTX33066 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX33066 CBFb antibody.
Dilution : 1:100

Anti-CBFb antibody used in IHC (Paraffin sections) (IHC-P). GTX33066

GTX33066 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX33066 CBFb antibody.
Dilution : 1:200

REFERENCE

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REVIEW

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Package List Price ($)
$ 329