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CBL antibody [3B12]

Anti-CBL antibody [3B12] used in ELISA (ELISA). GTX60481
Anti-CBL antibody [3B12] used in IHC (Paraffin sections) (IHC-P). GTX60481
Anti-CBL antibody [3B12] used in Flow cytometry (FACS). GTX60481
Anti-CBL antibody [3B12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60481
Anti-CBL antibody [3B12] used in Western Blot (WB). GTX60481
Anti-CBL antibody [3B12] used in IHC (Paraffin sections) (IHC-P). GTX60481

Cat No. GTX60481

Host

Mouse

Clonality

Monoclonal

Clone Name

3B12

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites, 0.03% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human C-CBL expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cbl proto-oncogene , C-CBL , CBL2 , FRA11B , NSLL , RNF55

Cellular Localization

Cytoplasm,Cell membrane,Golgi apparatus

Background

This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-CBL antibody [3B12] used in ELISA (ELISA). GTX60481

GTX60481 ELISA Image

ELISA analysis of antigen using GTX60481 CBL antibody [3B12].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-CBL antibody [3B12] used in IHC (Paraffin sections) (IHC-P). GTX60481

GTX60481 IHC-P Image

IHC-P analysis of ovarian cancer tissue using GTX60481 CBL antibody [3B12].

Anti-CBL antibody [3B12] used in Flow cytometry (FACS). GTX60481

GTX60481 FACS Image

FACS analysis of MCF-7 cells using GTX60481 CBL antibody [3B12].
Blue : CBL
Red : negative control

Anti-CBL antibody [3B12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60481

GTX60481 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60481 CBL antibody [3B12].
Green : CBL
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-CBL antibody [3B12] used in Western Blot (WB). GTX60481

GTX60481 WB Image

WB analysis of Raji (1), Raw264.7 (2),K562 (3), SKBR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate using GTX60481 CBL antibody [3B12].

Anti-CBL antibody [3B12] used in IHC (Paraffin sections) (IHC-P). GTX60481

GTX60481 IHC-P Image

IHC-P analysis of bladder cancer tissue using GTX60481 CBL antibody [3B12].

REFERENCE

There are currently no references for CBL antibody [3B12] (GTX60481). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CBL antibody [3B12] (GTX60481). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 339