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CBL antibody [C2C3], C-term

Anti-CBL antibody [C2C3], C-term used in Western Blot (WB). GTX101352
Anti-CBL antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101352

Cat No. GTX101352

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:50-1:200
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

THP-1 , *293T

Predict Reactivity

Mouse, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CBL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cbl proto-oncogene , C-CBL , CBL2 , FRA11B , NSLL , RNF55

Cellular Localization

Cytoplasm

Background

The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-CBL antibody [C2C3], C-term used in Western Blot (WB). GTX101352

GTX101352 WB Image

CBL antibody [C2C3], C-term detects CBL protein by Western blot analysis.
A. 30 µg THP-1 whole cell lysate/extract
7.5 % SDS-PAGE
CBL antibody [C2C3], C-term (GTX101352) dilution: 1:1000

Anti-CBL antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101352

GTX101352 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using CBL(GTX101352) antibody at 1:50 dilution.

REFERENCE
There are currently no references for CBL antibody [C2C3], C-term (GTX101352). Be the first to share your publications with this product.
REVIEW

CBL antibody [C2C3], C-term Cat No. GTX101352

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 31-Oct-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169