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CBL antibody [C2C3], C-term

Anti-CBL antibody [C2C3], C-term used in Western Blot (WB). GTX101352
Anti-CBL antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101352

Cat No. GTX101352

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:50-1:200
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

THP-1 , *293T

Predict Reactivity

Mouse, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CBL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036459

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cbl proto-oncogene , C-CBL , CBL2 , FRA11B , NSLL , RNF55

Cellular Localization

Cytoplasm

Background

The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CBL antibody [C2C3], C-term used in Western Blot (WB). GTX101352

GTX101352 WB Image

CBL antibody [C2C3], C-term detects CBL protein by Western blot analysis.
A. 30 μg THP-1 whole cell lysate/extract
7.5 % SDS-PAGE
CBL antibody [C2C3], C-term (GTX101352) dilution: 1:1000

Anti-CBL antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101352

GTX101352 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using CBL(GTX101352) antibody at 1:50 dilution.

REFERENCE

There are currently no references for CBL antibody [C2C3], C-term (GTX101352). Be the first to share your publications with this product.

REVIEW

CBL antibody [C2C3], C-term Cat No. GTX101352

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 31-Oct-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169