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CBL antibody

Anti-CBL antibody used in Western Blot (WB). GTX31301
Anti-CBL antibody used in IHC (Paraffin sections) (IHC-P). GTX31301

Cat No. GTX31301

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 2 μg/mL
IHC-P 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

Human Lymphoid Tissue Tissue Slide(GTX21833) , Daudi Cell Lysate(GTX23951) , Human Lymph node Tissue Lysate(GTX29055)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Cbl antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human cbl.The immunogen is located within amino acids 810 - 860 of Cbl.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cbl proto-oncogene , C-CBL , CBL2 , FRA11B , NSLL , RNF55

Cellular Localization

Cytoplasm,Cell membrane,Golgi apparatus

Background

This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-CBL antibody used in Western Blot (WB). GTX31301

GTX31301 WB Image

WB analysis of Daudi cell lysate using GTX31301 CBL antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

Anti-CBL antibody used in IHC (Paraffin sections) (IHC-P). GTX31301

GTX31301 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX31301 CBL antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369